摘要
该实验采用自组装法制备及表征三七总皂苷壳聚糖纳米粒(Panax notoginseng saponins chitosan nanoparticles,PNS-NPs),对其外观、粒径、包封率、载药量、多分散指数(PDI)、Zeta电位、微观结构等进行表征。所制得PNS-NPs形态结构完整,平均粒径为(209±0.258)nm、包封率为42.34%±0.28%、载药量为37.63%±0.85%、Zeta电位为(39.8±3.122)mV。之后对PNS-NPs大鼠肠吸收特性进行研究,建立三七总皂苷的HPLC分析方法,对pH、灌流速度、不同药物(三七总皂苷原料药、血塞通胶囊、PNS-NPs)进行考察,计算并分析在十二指肠段、空肠段、回肠段、结肠段的吸收速率常数(K_(a))与表观渗透系数(P_(app))。结果表明灌流液pH为6.8时,PNS-NPs在大鼠小肠内的吸收相较于其他pH显著提高(P<0.05),灌流速度对PNS-NPs的K_(a)和P_(app)没有显著影响,PNS-NPs较三七总皂苷原料药、血塞通胶囊在小肠内的吸收结果存在显著性差异(P<0.05),且PNS-NPs在小肠中的吸收显著提高。
In this experiment,Panax notoginseng saponins chitosan nanoparticles(PNS-NPs)were prepared by self-assembly and their appearance,particle size,encapsulation efficiency,drug loading,polydispersity index(PDI),Zeta potential,and microstructure were characterized.The prepared PNS-NPs were intact in structure,with an average particle size of(209±0.258)nm,encapsulation efficiency of 42.34%±0.28%,a drug loading of 37.63%±0.85%,and a Zeta potential of(39.8±3.122)mV.The intestinal absorption of PNS-NPs in rats was further studied.The established HPLC method of PNS was employed to investigate the effects of pH,perfusion rate,and different drugs(PNS raw materials,Xuesaitong Capsules,and PNS-NPs).The absorption rate constant(K_(a))and apparent permeability coefficient(P_(app))in the duodenum,jejunum,ileum,and colon were calculated and analyzed.As illustrated by the results,the intestinal absorption of PNS-NPs was increased in the perfusion solution at pH 6.8(P<0.05),and perfusion rate had no significant effect on the K_(a)and P_(app)of PNS-NPs.The intestinal absorption of PNS-NPs was significantly different from that of PNS raw materials and Xuesaitong Capsules(P<0.05),and the intestinal absorption of PNS-NPs was significantly improved.
作者
徐鹏飞
张锐
关志宇
李思慧
周冬艳
姜晟
范瑾慧
徐彭
朱卫丰
XU Peng-fei;ZHANG Rui;GUAN Zhi-yu;LI Si-hui;ZHOU Dong-yan;JIANG Sheng;FAN Jin-hui;XU Peng;ZHU Wei-feng(School of Pharmacy,Jiangxi University of Chinese Medicine,Nanchang 330004,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2022年第1期95-102,共8页
China Journal of Chinese Materia Medica
基金
国家“重大新药创制”科技重大专项(2018ZX09721002-008)
国家重点研发计划中医药现代化研究重点专项(2017YFC1702904)
江西省中医药管理局科技计划项目(2020B0366)
江西省博士后研究人员科研项目(2020KY51)。
关键词
三七总皂苷
壳聚糖
口服纳米粒
表征
肠吸收
Panax notoginseng saponins(PNS)
chitosan
oral nanoparticles
characterization
intestinal absorption
