摘要
采用超滤对牡蛎酶解液(oyster enzymatic hydrolysate,OE)进行分级,获得OE-Ⅰ(<5 kDa)、OE-Ⅱ(5~10 kDa)与OE-Ⅲ(>10 kDa)3个多肽组分。以1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基清除能力、羟自由基清除能力、超氧阴离子自由基清除能力和亚铁离子螯合能力为指标,评价牡蛎多肽3个组分的体外抗氧化活性。以秀丽隐杆线虫为模型,研究不同剂量牡蛎多肽组分OE-Ⅰ对线虫寿命、急性氧化应激、热应激、体内抗氧化酶活力、体内活性氧和细胞凋亡的影响,测定线虫寿命相关基因的表达水平并分析其抗衰老机制。结果发现,牡蛎多肽组分OE-Ⅰ的抗氧化活性最强,且与空白组相比,OE-Ⅰ高剂量组线虫的寿命极显著延长(P<0.01);线虫体内3种主要抗氧化酶中超氧化物歧化酶活力显著提高(P<0.05),过氧化氢酶和谷胱甘肽过氧化物酶活力极显著提高(P<0.01),线虫体内活性氧的积累和细胞凋亡减少;此外,组分OE-Ⅰ通过上调skn-1、daf-16、sod-3和mtl-1基因的表达,下调daf-2和age-1基因的表达,提高了线虫的应激抵抗能力。综上,牡蛎多肽组分OE-I可作为抗氧化和抗衰老的功能性食品基料。
Oyster enzymatic hydrolysate(OE) was fractionated by ultrafiltration into three fractions: OE-Ⅰ(< 5 kDa),OE-Ⅱ(5–10 kDa) and OE-Ⅲ(> 10 kDa). The in vitro antioxidant activities of the fractions were evaluated in terms of their 1,1-diphenyl-2-picrylhydrazyl(DPPH), hydroxyl, and superoxide anion radical scavenging capacity and ferrous ion chelating capacity. Then, the effect of different doses of OE-Ⅰ on the lifespan, acute oxidative stress, thermal stress,antioxidant enzyme activities, reactive oxygen species(ROS) accumulation and cell apoptosis of Caenorhabditis elegans was investigated, and the expression levels of lifespan-related genes were measured to unravel the anti-aging mechanism of OE-Ⅰ. The results showed that OE-Ⅰ had the strongest antioxidant activity among the three fractions, and that high-dose of OE-Ⅰ significantly prolonged the lifespan of C. elegans(P < 0.01). The activities of superoxide dismutase(SOD)(P < 0.05),catalase(CAT) and glutathione peroxidase(GSH-Px)(P < 0.01) were significantly increased and the accumulation of ROS and cell apoptosis were decreased. In addition, the expression of the skn-1, daf-16, sod-3 and mtl-1 genes were up-regulated,while the expression of the daf-2 and age-1 genes were down-regulated, suggesting improved stress resistance of C. elegans.To sum up, OE-Ⅰ can be used as a functional food base material for anti-oxidation and anti-aging.
作者
王力
肖嵋方
陈弘培
刘斌
曾峰
WANG Li;XIAO Meifang;CHEN Hongpei;LIU Bin;ZENG Feng(Engineering Research Centre of Fujian-Taiwan Special Marine Food Processing and Nutrition,Ministry of Education,Fuzhou 350002,China;College of Food Science,Fujian Agriculture and Forestry University,Fuzhou 350002,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2022年第3期152-160,共9页
Food Science
基金
福建省科技重大专项(2020NZ012010)
厦门海洋科技成果转化与产业化示范项目(18CYY026HJ13)。
