摘要
目的初步探讨外周血CD4^(+)CD25^(+)调节性T(Treg)细胞在妊娠合并原发免疫性血小板减少症(ITP)中的作用。方法选取2020年1-12月在北部战区总医院就诊的妊娠合并ITP患者100例作为试验组,其中急性ITP患者36例、慢性ITP患者64例,对照组为100例年龄、孕周相仿的正常产检孕妇。采用流式细胞术(FCM)检测两组外周血中CD4^(+)T细胞及CD4^(+)CD25^(+)Treg细胞的水平。结果试验组CD4^(+)T细胞、CD4^(+)CD25^(+)Treg细胞及CD4^(+)CD25^(+)Treg细胞/CD4^(+)T细胞比值均低于对照组,差异均有统计学意义(P<0.05)。急性ITP患者的CD4^(+)T细胞和CD4^(+)CD25^(+)Treg细胞低于慢性ITP患者,差异均有统计学意义(P<0.05);急性ITP患者的CD4^(+)CD25^(+)Treg细胞/CD4^(+)T细胞比值低于慢性ITP患者,但差异无统计学意义(P>0.05)。试验组CD4^(+)CD25^(+)Treg细胞与血小板计数呈较强正相关(r=0.759,P<0.001),CD4^(+)CD25^(+)Treg细胞/CD4^(+)T细胞比值与血小板计数呈中等程度正相关(r=0.539,P<0.001)。结论妊娠合并ITP患者外周血中CD4^(+)CD25^(+)Treg细胞水平下降,并且急性ITP时下降更明显,同时CD4^(+)CD25^(+)Treg细胞与血小板计数呈正相关。
Objective To investigate the role of peripheral blood CD4^(+)CD25^(+) regulatory T(Treg)cells in pregnancy complicating primary immune thrombocytopenia(ITP).Methods A total of 100 patients with pregnancy complicating ITP admitted to this hospital from January 2020 to December 2020 were selected as the experimental group,including 36 cases of acute ITP and 64 cases of chronic ITP.The control group included 100 normal antenatal care pregnant women with similar age and gestational age.The levels of CD4^(+)T and CD4^(+)CD25^(+) Treg cells in peripheral blood of the two groups were measured by the flow cytometry(FCM).Results The levels of CD4^(+)T cells,CD4^(+)CD25^(+) Treg cells and CD4^(+)CD25^(+) Treg cells/CD4^(+)T cells ratio in the experimental group were lower than those in the control group and the differences were statistically significant(P<0.05).The levels of CD4^(+)T and CD4^(+)CD25^(+) Treg cells in the patients with acute ITP were lower than those in the patients with chronic ITP,and the differences were statistically significant(P<0.05);the ratio of CD4^(+)CD25^(+) Treg cells/CD4^(+)T cells ratio in the patients with acute ITP was lower than that in the patients with chronic ITP,and the difference was not statistically significant(P>0.05).There was a strong positive correlation between CD4^(+)CD25^(+) Treg cells and platelet count in the experimental group(r=0.759,P<0.001),and the CD4^(+)CD25^(+) Treg cells/CD4^(+)T cells ratio had a moderate positive correlation with the platelet count(r=0.539,P<0.001).Conclusion The expression level of CD4^(+)CD25^(+) Treg cells in peripheral blood of the patients with pregnancy complicating ITP is decreased,moreover the decrease in acute ITP is more obvious,meanwhile the CD4^(+)CD25^(+)Treg cells are positively correlated with the platelet count.
作者
李明亮
田笑
马昂
栾亮
刘静
刘婷婷
LI Mingliang;TIAN Xiao;MA Ang;LUAN Liang;LIU Jing;LIU Tingting(Department of Clinical Laboratory,General Hospital of Northern Theater Command,Shenyang,Liaoning 110016,China)
出处
《检验医学与临床》
CAS
2022年第4期459-462,共4页
Laboratory Medicine and Clinic
基金
辽宁省自然科学基金项目(20180551178)。
