摘要
每年重大病虫害的流行和暴发都会对农业产生重大的影响,进而造成严重的经济损失,随着化学试剂的施用其抗药性也逐渐增强,因此探索新的绿色、安全、高效的害虫防治技术具有十分重要的意义。RNAi作为一种研究基因功能的工具,在开发新的害虫防治策略方面显示出巨大的潜力,但RNAi的效率受限于核酸酶的降解作用,为了改善dsRNA的有效递送,研发了纳米载导技术且被广泛应用于害虫防治。同时,基于工程菌高效合成靶向昆虫dsRNA的技术为田间推广提供了可行性及技术支撑。本文主要对RNAi技术及纳米载导RNAi技术在害虫防治中的研究和应用进行了总结和归纳。
Each year,the epidemic and outbreak of major diseases and pests will have a major impact on agriculture,resulting in serious economic losses,with the application of chemical reagents its resistance has gradually increased,therefore,it is of great significance to explore new green,safe and efficient pest control technology.RNAi,as a tool to study gene function,has shown great potential in developing new pest control strategies.However,the efficiency of RNAi is limited by the degradation effect of nuclease.In order to improve the effective delivery of dsRNA,nanoparticle-mediated technology was developed and widely used in pest control.At the same time,the efficient synthesis of targeted insect dsRNA based on engineered bacteria provides feasibility and technical support for field promotion.This paper mainly summarized the research and application of RNAi technology and nanoparticle-mediated technology in pest control.
作者
王晓迪
冀顺霞
申晓娜
刘万学
万方浩
张桂芬
吕志创
WANG Xiaodi;JI Shunxia;SHEN Xiaona;LIU Wanxue;WAN Fanghao;ZHANG Guifen;Lü Zhichuang(State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100193,China)
出处
《中国生物防治学报》
CSCD
北大核心
2021年第6期1298-1312,共15页
Chinese Journal of Biological Control
基金
国家自然科学基金(32072494,31672088)
国家重点研发计划(2017YFC1200600)。
