摘要
目的探讨基于氨基末端激酶(JNK)/p38通路调控微小RNA-7(miR-7)对幽门螺杆菌(Hp)感染诱导的结直肠癌大鼠的干预作用。方法选取60只雄性SD大鼠,15只作为正常组,其余45只建立Hp感染诱导的结直肠癌模型,并随机分为模型组、上调组、下调组各15只。上调组、下调组做miR-7转染。鉴定miR-7转染效率,比较每组检出率,测定Th17/Treg相关因子mRNA表达水平及JNK/p38通路蛋白表达量。结果与正常组相比,其他三组miR-7、Foxp3、IL-10表达较低,IL-17A、IL-21、JNK、p38表达较高(P<0.05);与模型组相比,下调组miR-7、Foxp3、IL-10表达较低,IL-17A、IL-21、JNK、p38表达较高,上调组miR-7、Foxp3、IL-10表达较高,IL-17A、IL-21、JNK、p38表达较低(P<0.05)。结论上调miR-7可改善机体的免疫能力,减缓结直肠癌的发生进程,其作用机制可能与JNK/p38通路有关。
Objective To explore the intervention effect of microRNA-7(miR-7) regulated by amino-terminal kinase(JNK)/p38 pathway on colorectal cancer rats induced by helicobacter pylori(Hp) infection. Methods Sixty male SD rats were selected, 15 of them were taken as the normal group, and the other 45 rats were used to establish colorectal cancer models induced by Hp infection, which were randomly divided into model group, up-regulated group and down-regulated group, with 15 rats in each group. The up-regulated group and down-regulated group were transfected with miR-7. The transfection efficiency of miR-7 was identified, the detection rate of each group was compared, and the expression level of Th17/Treg related factors mRNA and JNK/p38 pathway protein were measured. Results Compared with the normal group, the expressions of miR-7, Foxp3, IL-10 were lower in the other three groups, and IL-17 A, IL-21,JNK and p38 were higher in the other three groups(P<0.05). Compared with the model group, the expressions of miR-7,Foxp3 and IL-10 were lower in the down-regulated group, while the expressions of IL-17 A, IL-21, JNK and p38 were higher. The expressions of miR-7, Foxp3 and IL-10 were higher in the up-regulated group, while the expression of IL-17 A, IL-21, JNK and p38 was lower(P<0.05). Conclusion Up-regulated miR-7 can improve immunity and slow down the development of colorectal cancer, and its mechanism may be related to JNK/p38 pathway.
作者
潘崇南
王星钧
金辉
PAN Chongnan;WANG Xingjun;JIN Hui(Department of Infectious Diseases,Taizhou First People′s Hospital in Zhejiang Province,Taizhou 318020,China;Department of Respiratory Medicine,Taizhou First People′s Hospital in Zhejiang Province,Taizhou 318020,China;Department of Gastroenterology,Taizhou First People′s Hospital in Zhejiang Province,Taizhou 318020,China)
出处
《中国现代医生》
2021年第35期24-26,31,F0003,共5页
China Modern Doctor
基金
浙江省医药卫生科技计划项目(2020KY1044)。
