摘要
目的探讨饮食限制在锰诱导神经毒作用损伤过程中的作用及机制。方法40只成年昆明小鼠雌雄各半,随机分为4组:对照组、饮食限制组、锰暴露组和饮食限制锰暴露组。每周测量体重、观察行为学改变、检测血糖值,取小鼠脑组织计算脑脏器系数、检测脑内锰蓄积量、分析脑内炎症因子水平并检测相关蛋白表达。结果与对照组比较,锰暴露组小鼠行为学发生异常改变,脑系数下降,白细胞介素4(IL-4)下降,肿瘤坏死因子–α(TNF-α)和诱导型一氧化氮合酶(iNOS)、核因子–κB(NF-κB)、caspase-8升高(P<0.05),引起炎症反应;与锰暴露组比较,饮食限制锰暴露组小鼠脑脏器系数下降、TNF-α、iNOS含量下降,NF-κB、caspase-8表达水平降低(P<0.05)。结论饮食限制在锰诱导神经毒作用损伤中具有一定的保护作用;其机制可能与其抑制炎症反应、降低凋亡水平、增强自噬作用有关。
Objective To explore the effect and mechanism of dietary restriction(DR)on manganese(Mn)-induced neurotoxicity.Methods Forty adult Kunming mice(male:female=1:1)were randomly divided into four groups(10 in each group):control group(25.0 g feed/day and intraperitoneal injection of saline),DR control group(12.5 g feed/day and intraperitoneal injection of saline),Mn exposure group(25.0 g feed/day and intraperitoneal injection of Mn at dose of 200μmol/kg)and DR intervention group(12.5 g feed/day and intraperitoneal injection of Mn at dose of 200μmol/kg).The treatments were conducted once a day continuously for 6 weeks.The mice’s weight and blood glucose were measured and their behavioral changes were observed every week.By the end of the treatments,the brain specimens were collected for determinations of brain coefficient,cumulated Mn content,levels of inflammatory factors,and expressions of related proteins.Results In the Mn exposed mice,abnormal behavioral changes were observed;decreased brain coefficient and interleukin 4(IL-4)but increased tumor necrosis factor-α(TNF-α),inducible nitric oxide synthase(iNOS),protein expression of nuclear factor kappa B(NF-κB),and caspase-8 were detected compared to those in the control mice(all P<0.05).Decreased brain coefficient,TNF-α,iNOS,NF-κB,and caspase-8 were detected in the Mn exposed mice with DR in comparison with those in the Mn exposed mice with normal feeding(P<0.05 for all).Conclusion Dietary restriction could play a protective role in Mn-induced neurotoxicity and the effect may be related to inflammation suppression,apoptosis reduction,and autophagy enhancement in mice.
作者
郎静
高亮
王文博
高显会
马洪林
闫冬莹
LANG Jing;GAO Liang;WANG Wen-bo(Department of Occupational and Environmental Health,School of Public Health,Jinzhou Medical University,Jinzhou,Liaoning Province 121001,China)
出处
《中国公共卫生》
CSCD
北大核心
2021年第12期1793-1796,共4页
Chinese Journal of Public Health
基金
辽宁省自然科学基金计划项目(2020–BS–248)。
关键词
饮食限制
锰
神经毒性
炎症
凋亡
自噬
dietary restriction
manganese
neurotoxicity
inflammation
apoptosis
autophagy
