摘要
目的应用去势联合腹腔注射地塞米松法建立大鼠肌少-骨质疏松症模型并确定本病的评价指标。方法将40只6月龄SPF级雌性SD大鼠随机分为正常组、假手术组、假手术+地米组、去势组、去势+地米组,每组8只。假手术组、假手术+地米组大鼠行假手术,去势组、去势+地米组大鼠行双侧卵巢切除术。术后一周,假手术+地米组、去势+地米组大鼠腹腔注射地塞米松,连续二周。造模后3个月,各组大鼠分别进行抓力测定、全身和股骨全段骨密度测定、骨骼肌质量指数和相对骨骼肌质量指数测定、股骨远端骨微结构测定等,并探讨本病的评价指标。结果①与假手术组相比,假手术+地米组大鼠前肢抓力、骨骼肌质量指数和相对骨骼肌质量指数明显降低。②与假手术组相比,去势组和去势+地米组大鼠前肢抓力、骨骼肌质量指数和相对骨骼肌质量指数明显降低,全身骨密度、股骨全段骨密度、股骨远端骨体积分数、骨表面密度和骨小梁数明显下降,骨小梁分离度明显提高。③与去势组相比,去势+地米组大鼠前肢抓力有下降趋势、骨骼肌质量指数和相对骨骼肌质量指数明显降低。结论去势联合腹腔注射地塞米松法可以建立肌少-骨质疏松症大鼠模型,这种复合造模法具有造模周期短、模型稳定、可操作性强、成本低等优点。大鼠前肢抓力、骨骼肌质量指数和相对骨骼肌质量指数、全身骨密度和股骨骨密度、股骨远端骨微结构等指标可以作为本病的评价指标。
Objective To establish a rat model of osteosarcopenia(OS)with ovariectomy(OVX)combined with intraperitoneal injection of dexamethasone(DXM),and to determine the evaluation index of the disease.Methods Forty 6-month-old SPF female SD rats were randomly divided into Control group,Sham group,Sham+DXM group,OVX group,and OVX+DXM group,with 8 rats in each group.Rats in Sham group and Sham+DXM group underwent sham operation,while rats in OVX group and OVX+DXM group underwent bilateral ovariectomy.One week after the operation,rats in Sham+DXM group and OVX+DXM group were injected with DXM intraperitoneally for two weeks.Three months after the establishment of the model,the rats in each group were measured for grip,bone mineral density(BMD)of the whole body and the femur,skeletal muscle mass index(SMI)and relative skeletal muscle mass index(RSMI),bone microstructure of the distal femur.The evaluation indexes of the disease were discussed.Results(1)Compared with the Sham group,the rats in the Sham+DXM group had significantly lower forelimb grip,SMI,and RSMI.(2)Compared with those in the Sham group,forelimb grip,SMI and RSMI,the whole body BMD,the whole femur BMD,the distal femur bone volume fraction,the bone surface density,and the number of bone trabeculae in both the OVX group and the OVX+DXM group decreased significantly,and the separation of bone trabeculae increased significantly.(3)Compared with those in the OVX group,the forelimb grip strength of rats showed a downward trend,SMI and RSMI of the OVX+DXM group reduced significantly.Conclusion OVX combined with intraperitoneal injection of DXM can be used to establish the OS rat model,which has the advantages of short modeling cycle,stable model,strong availability,and low cost.The grip of the forelimb,SMI and RSMI,BMD of the whole body,BMD of the femur,and the bone microstructure of the distal femur can be used as the evaluation indexes of the disease.
作者
马江涛
黄佳纯
黄润龙
万雷
黄红
黄宏兴
MA Jiangtao;HUANG Jiachun;HUANG Runlong;WAN Lei;HUANG Hong;HUANG Hongxing(Guangzhou University of Chinese Medicine,Guangzhou 510405;Laboratory of Orthopedics and Traumatology of Chinese Medicine,Lingnan Medical Research Center of Guangzhou University of Chinese Medicine,Guangzhou 510006;The Third Affiliated Hospital of Guangzhou University of Chinese Medicine,Guangzhou 510375;College of Nursing,Guangzhou University of Chinese Medicine,Guangzhou 510006,China)
出处
《中国骨质疏松杂志》
CAS
CSCD
北大核心
2022年第1期1-5,15,共6页
Chinese Journal of Osteoporosis
基金
国家自然科学基金面上项目(81973886)
广州中医药大学学科研究重点项目(XK2019028)。
