摘要
PR1是植物病程相关(Pathogenesis related,PR)蛋白家族成员之一,参与植物抗病防御反应。研究前期明确了小麦TaPR1基因受叶锈菌及信号分子水杨酸(Salicylic acid,SA)、脱落酸(Abscisic acid,ABA)的诱导表达。本研究基于中国春小麦数据库,克隆获得了小麦TaPR1基因上游2200 bp启动子序列,对启动子区域所包含的顺式作用元件进行分析预测,利用β-葡萄糖苷酸酶(β-Glucuronidase,GUS)组织化学染色、绿色荧光蛋白(Green fluorescent protein,GFP)观察对不同长度启动子区段进行启动活性验证,结果表明TaPR1基因启动子-2200~-290 bp区段具有启动活性,为进一步解析TaPR1基因转录调控机制提供了理论依据。
PR1 is a member of pathogenesis related(PR)protein family of plants and participates in the defense response of plant disease resistance.In our previous study,we found that wheat TaPR1 gene was induced by signal molecules salicylic acid(SA)and abscisic acid(ABA).In this study,the sequence of 2200 bp upstream of TaPR1 gene was obtained from genome database of Chinese Spring followed by cis-acting elements prediction.Then different fragments of the promoter were cloned for activity analysis usingβ-glucuronidase(GUS)and green fluorescent protein(GFP)fluorescence reporters.The-2200--290 bp region showed activation activity.These results will lay theoretical foundation for the study of regulation mechanism of TaPR1.
作者
王丽珊
王珅
王菲
王海燕
刘大群
WANG Lishan;WANG Shen;WANG Fei;WANG Haiyan;LIU Daqun(College of Plant Protection,Hebei Agricultural University/Biological Control Technological Innovation Center of Plant Diseases and Plant Pests of Hebei province,Baoding 071000,China;Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences/Hebei Laboratory of Crop Genetic and Breeding,Shijiazhuang 050035,China)
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2022年第1期7-11,68,共6页
Journal of Hebei Agricultural University
基金
河北省自然科学基金项目(C2020204028)
国家自然科学基金项目(32172384).
