摘要
为探讨虫草菌株胞内、胞外多糖体外抗氧化和免疫活性及其量效关系,采用水提醇沉法、sevege法和活性炭吸附法,分别对大规模发酵生产获得的虫草菌丝体及发酵液2种不同生产原料进行多糖提取,重点分析其胞内及胞外多糖体外抗氧化和免疫活性。结果表明:虫草多糖最佳提取工艺为液料比95∶1 (mL/g)、提取温度65℃、提取时间1.5 h;多糖中含少量的核酸和蛋白质,是以β-糖苷键连接且具有吡喃糖环的酸性杂多糖;分离纯化自发酵液及发酵虫草菌粉中的多糖均具有较好的清除自由基能力且呈一定的量效关系;2种多糖样品均能促进RAW 264.7巨噬细胞增殖,促进细胞释放NO,且发酵液多糖活性优于胞内多糖,与LPS组具有显著性差异(P<0.001)。发酵虫草菌粉及发酵液提取的多糖在体外活性研究中均呈现出较好的抗氧化活性及免疫活性,有望开发成为新型药物免疫调节剂或天然抗氧化剂等新资源功能食品,也为发酵虫草菌粉胞内多糖和发酵液利用及产品开发提供了科学依据。
In order to explore the in vitro antioxidant and immune activities of the intracellular and extracellular C. sinensis polysaccharides and their dose-effect relationship, polysaccharides were extracted from C. mycelium and fermentation broth by water extraction and alcohol precipitation method, sevege method and activated carbon adsorption method, respectively. The antioxidant and immune activities of intracellular and extracellular polysaccharides were analyzed in vitro. The results showed that the optimal extraction conditions were liquid/solid ratio 95∶ 1 mL/g, extraction temperature 65 ℃ and extraction time 1.5 h. The results of UV and IR spectra of C. sinensis polysaccharides showed that the polysaccharide samples contained nucleic acids and proteins in different degrees, and were acidic heteropolysaccharides with β-glycoside bonds and pyranose rings. This study also confirmed that the polysaccharides from the self-fermentation liquid and the fermented cordyceps powder had a good free radical scavenging ability and showed a certain dose-effect relationship. The effects of polysaccharides on immune cells were further compared, and it was found that both polysaccharides could promote the proliferation of RAW 264.7 macrophages and promote the release of NO, and the activity of polysaccharides in fermentation broth was higher than that of intracellular polysaccharides, and there was a significant difference with LPS group(P< 0.001). Therefore, the fermented liquor collected from C. sinensis polysaccharides extract showed good antioxidant activity and immune activity in vitro, which is expected to develop as new drugs to improve immunity or new functional food resources, such as natural antioxidant.
作者
王兰英
姜玲
赵静
李绍平
WANG Lanying;JIANG Ling;ZHAO Jing;LI Shaoping(School of Pharmacy and Food Sciences,Zhuhai College of Science and Technology,Zhuhai 519041,China;The State Key Laboratory for Quality Research in Chinese Medicine,University of Macao,Macao 999078,China)
出处
《河南工业大学学报（自然科学版）》
CAS
北大核心
2021年第6期80-88,共9页
Journal of Henan University of Technology：Natural Science Edition
基金
广东省创新强校工程项目(2019KTSCX222,2019KQNCX200)。
关键词
冬虫夏草
发酵虫草菌粉
多糖
抗氧化活性
免疫活性
C.sinensis
fermented C.sinensis powder
polysaccharides
antioxidant activity
immune activity
