摘要
目的探究在乙肝相关肝细胞癌复发中转录中介因子1γ(TIF1γ)、MicroRNA和乙肝病毒基因(HBV-DNA)的表达情况。方法把2017年1月—2020年12月该院接收的78例乙肝相关肝细胞癌患者作为观察组,同期另选取非复发乙肝相关肝细胞癌患者78例作为对照组。实施聚合酶链式反应(PCR)技术对乙肝相关肝细胞癌肝脏组织及血液样本中HBV-DNA、TIF1γ和MicroRNA表达情况进行检测。结果在比较TIF1γ、MicroRNA表达水平中,观察组的低表达水平分别是(0.49±0.06)、(0.52±0.14),均高于对照组的(0.01±0.02)、(0.01±0.06),差异有统计学意义(t=67.028、29.572,P<0.05)。复发手术组患者TIF1γ、MicroRNA低表达水平分别是(0.67±0.09)、(0.59±0.12),明显高于复发非手术组的(0.32±0.05)、(0.48±0.11),差异有统计学意义(t=21.623、4.222,P<0.05)。复发手术组术前急性期、术后3 d、术后2周HBV-DNA水平阳性率占比分别是86.11%、80.56%、41.67%,明显高于对照组的12.82%、17.95%、1.28%,差异有统计学意义(χ^(2)=57.450、41.098、33.296,P<0.05)。结论在乙肝相关肝细胞癌患者中,诱发乙肝相关肝细胞癌复发的因素主要为HBV-DNA阳性表达及TIF1γ、MicroRNA低表达水平增高,且能对乙肝相关细胞癌患者预后带来影响。
Objective To investigate the expression of transcription intermediary factor 1γ(TIF1γ),MicroRNA and hepatitis B virus gene(HBV-DNA)in the recurrence of hepatitis B-related hepatocellular carcinoma.Methods The 78 patients with hepatitis B-related hepatocellular carcinoma accepted by the hospital from January 2017 to December 2020 were taken as the observation group.In the same period,78 patients with non-recurring hepatitis B-related hepatocellular carcinoma were selected as the control group.Implement polymerase chain reaction(PCR)technology to detect the expression of HBV-DNA,TIF1γ and MicroRNA in hepatitis B-related hepatocellular carcinoma liver tissue and blood samples.Results In comparison of the expression levels of TIF1γ and MicroRNA,the low expression levels of TIF1γ and MicroRNA in the observation group were(0.49±0.06)and(0.52±0.14),respectively,which were higher than those in the control group(0.01±0.02)and(0.01±0.06),the difference was statistically significant(t=67.028,29.572,P<0.05).The low expression levels of TIF1γ and MicroRNA in recurrent surgery group were(0.67±0.09)and(0.59±0.12),respectively,which were significantly higher than those in non-recurrent surgery group(0.32±0.05)and(0.48±0.11),the difference was statistically significant(t=21.623,4.222,P<0.05).The proportion of positive rate of HBV-DNA level of acute period before surgery,3 d after surgery and 2 weeks after surgery in the recurrence surgery group was 86.11%,80.56%,41.67%,significantly higher than 12.82%,17.95%,1.28% of the control group,the difference was statistically significant(χ^(2)=57.450,41.098,33.296,P<0.05).Conclusion In patients with hepatitis B-related hepatocellular carcinoma,the main factors that induce the recurrence of hepatitis B-related hepatocellular carcinoma are positive expression of HBV-DNA and the increase of low expression level of TIF1γ and MicroRNA,which can affect the prognosis of patients with hepatitis B-related cell carcinoma.
作者
臧唯
索朗多吉
旦增群培
ZANG Wei;Suolangduoji;Danzengqunpei(Department of Hepatobiliary Surgery,Jilin Central Hospital City,Jilin,Jilin Province,132000 China;Department of Surgery,Dingjie County People's Hospital,Shigatse,Dingjie,Tibet Autonomous Region,857900 China)
出处
《系统医学》
2021年第21期5-8,共4页
Systems Medicine
基金
吉林省卫生计生委科研计划(2014Q002)。
