摘要
[目的]筛选林芝云杉球果挥发物中对冷杉梢斑螟有引诱作用的物质,以期明确冷杉梢斑螟寄主选择机制,从而为该害虫的无公害防治提供理论依据。[方法]采用触角电位仪(EAG)和"Y"型嗅觉仪对林芝云杉球果挥发物中主要成分不同浓度及不同配方分别进行EAG反应和嗅觉行为测试。GC-MS结果表明:健康新果和虫害旧果均含有6种化合物,但其组分及含量存在差异。[结果]EAG反应结果表明:在10μg·μL^(-1)范围内,α-蒎烯、β-蒎烯、(1S)-(-)-β-蒎烯、柠檬烯各组分浓度越大,不同状态下冷杉梢斑螟成虫对其EAG反应越大,超过10μg·μL^(-1)后,EAG值反而减小;各组分浓度为10μg·μL^(-1)时,不同状态成虫EAG值显著高于其他浓度(P<0.05)。未交配雌、雄蛾对反式石竹烯的EAG反应随着浓度的增加而增大,在0.5μg·μL^(-1)内的EAG反应值与对照差异不显著(P>0.05),达到1μg·μL^(-1)以后,EAG值在各浓度间差异亦不显著(P>0.05),而0.5μg·μL^(-1)与1μg·μL^(-1)间EAG反应差异显著(P<0.05)。交配雌蛾、未交配雌蛾对月桂烯的EAG反应随浓度增大而增强,当浓度为100μg·μL^(-1)时,EAG值最大,但100μg·μL^(-1)与10μg·μL^(-1)的EAG值差异不显著(P>0.05)。[结论]嗅觉行为结果表明:配方A2、A3、A5、A7、A8对其具有明显引诱效果,尤以配方A7最佳,对不同状态下冷杉梢斑螟的引诱率达70%以上,配方A4、A9、A10对其具有明显趋避效果,对不同状态下冷杉梢斑螟的趋避率高达50%~70%。各组分的10μg·μL^(-1)为最佳刺激浓度,反式石竹烯具有明显引诱作用,月桂烯具有明显趋避作用。
[Objective]To screen the cone volatiles in the cones of Picea likiangensis var.linzhiensis,which have an attractive effect on the Dioryctria abietella,in order to clarify the host selection mechanism of D.abietella and provide a theoretical basis for the pest-free control of pests.[Method]Electroantennography(EAG)and"Y"olfactometer were used to measure the EAG reaction and olfactory behavior of D.abietella to the main components of the cone volatiles of P.likiangensis var.linzhiensis with different concentrations and formulations.[Result]The results of GC-MS showed that the healthy new cones and the infested old cones contained six compounds,but the composition and content were slightly different.The results of EAG showed that in the range of 10μg·μL^(-1),the higher the concentration ofα-pinene,β-pinene,(1 S)-(-)-β-pinene,limonene,the bigger the EAG response of the D.abietella in different states.However,the EAG value decreased after 10μg·μL^(-1).Under the concentration of 10μg·μL^(-1),the EAG value of the moth in different states was significantly higher than under the other concentrations(P<0.05).The EAG reaction of unmated female and male moths on beta-caryophyllene increased with the concentration,and the EAG reaction value in 0.5μg·μL^(-1) was not significantly different from the control(P>0.05).After reaching1μg·μL^(-1),no significant difference was found in the EAG value among concentrations(P>0.05),while the EAG reaction between 0.5μg·μL^(-1) and 1μg·μL^(-1) was significantly different(P<0.05).The EAG response of the mated female moth and the unmated female moth to myrcene increased with the concentration.When the concentration reached 100μg·μL^(-1),the EAG value was the largest,nevertheless the EAG value of 100μg·μL^(-1) and 10μg·μL^(-1) was not significantly different(P>0.05).The results of olfactory behavior showed that formula A2,A3,A5,A7 and A8 had obvious attracting effect,especially formula A7,and the attracting rate of D.abietella in different states was over70%.Unfortunately,formula A4,A9,A10 had obvious avoidance effect,and the avoidance rate of D.abietella in different states was as high as 50%~70%.[Conclusion]10μg·μL^(-1) of each component is the best stimulation concentration.Beta-caryophyllene has obvious attracting effect,while myrcene has obvious avoidance effect.
作者
唐晓琴
王思展
卢杰
高郯
陈羿渠
TANG Xiao-qin;WANG Si-zhan;LU Jie;GAO Tan;CHEN Yi-qu(Plant Sciences College,Tibet Agriculture&Animal Husbandry University,Nyingchi 860000,Tibet,China;Research Institute of Tibet Plateau Ecology,Tibet Agriculture&Animal Husbandry University,Nyingchi 860000,Tibet,China;Key Laboratory of Forest Ecology in Tibet Plateau(Tibet Agriculture&Animal Husbandry University),Ministry of Education,Nyingchi 860000,Tibet,China;Laboratory of Resource and Applied Insects in the Tibet Plateau,Nyingchi 860000,Tibet,China)
出处
《林业科学研究》
CSCD
北大核心
2021年第6期140-148,共9页
Forest Research
基金
国家自然科学基金项目(31460200)
西藏高原森林生态教育部重点实验室开放课题XZA-JYBSYS-2019-07。
关键词
冷杉梢斑螟
林芝云杉
球果挥发物
触角电位
行为反应
Dioryctria abietella
Picea likiangensis var.linzhiensis
cones volatiles
antenna potential
behavioral response
