摘要
目的观察活血化瘀法对慢性脑缺血大鼠神经元自噬及Aβ42生成的影响。方法采用改良后两步双侧颈总动脉结扎法建立慢性脑缺血大鼠模型,SD大鼠分为假手术组、模型组、脑得生组。用Morris水迷宫实验检测认知功能损伤程度,Western Blot法检测海马组织自噬相关蛋白Beclin-1、LC3-Ⅱ、p62及淀粉样前体蛋白(APP蛋白)表达水平,ELISA法检测海马组织Aβ42水平。结果与假手术组相比,模型组大鼠认知功能存在明显损伤,自噬相关蛋白Beclin-1、LC3-Ⅱ及p62表达显著升高(P<0.05,P<0.01),APP蛋白表达无变化(P>0.05),Aβ42水平显著升高(P<0.01);与模型组比较,脑得生组认知功能损伤减轻,自噬相关蛋白Beclin-1、LC3-Ⅱ及p62表达均显著降低(P<0.01,P<0.05),Aβ42表达显著降低(P<0.05)。结论活血化瘀法可通过抑制神经元自噬激活减少Aβ42生成,从而发挥改善慢性脑缺血大鼠认知功能的作用。
Objective To explore the effect of methods for activating blood circulation and removing blood stasis on autophagy and Aβ42 in hippocampus tissue of rats with chronic cerebral hypoperfusion.Methods The rat models of chronic cerebral hypoperfusion were made by two-step bilateral common carotid artery occlusion(BCCAO).SD rats were randomly divided into 3 groups: sham group, model group, and NDS group.Cognition(spatial memory)were evaluated by Morris water maze test.The protein expression of Beclin-1,LC3-Ⅱ,p62 and amyloid precursor protein(APP)were detected by Western Blot.Aβ42 level in hippocampus tissue were detected by ELISA.Results Compared with the sham group, the impairment of spatial memory appeared remarkably, the protein expression of Beclin-1,LC3-Ⅱ,p62 were all increased(P<0.05,P<0.01),while the expression of APP did not change(P>0.05)in model group, and the expression level of Aβ42 was increased in model group(P<0.01).Compared with the model group, the cognitive impairment in the NDS group was mildly damaged, the protein expression of Beclin-1,LC3-Ⅱ,p62 were all decreased(P<0.01,P<0.05),and the expression level of Aβ42 was decreased in NDS group(P<0.05).Conclusion The methods for activating blood circulation and removing blood stasis could decrease Aβ42 by inhibiting neuron autophagy, to improve cognitive impairment induced by chronic cerebral hypoperfusion.
作者
罗超琴
刘雪梅
吴艺帆
刘少姣
王凤丽
高俊峰
唐旭
巩颖
傅晨
LUO Chao-qin;LIU Xue-mei;WU Yi-fan;LIU Shao-jiao;WANG Feng-li;Gao Jun-feng;TANG Xu;GONG Ying;FU Chen(Beijing University of Chinese Medicine,Beijing 100029,China;Experimental Center of Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078;Department of Pharmacy,Dongfang Hospital,Beijing University of Chinese Medicine,Beijing 100078)
出处
《北京中医药》
2021年第10期1066-1070,共5页
Beijing Journal of Traditional Chinese Medicine
基金
国家自然科学基金资助项目(81973788)
国家中医药管理局中医药传承与创新“百千万”人才工程(岐黄学者)——国家中医药领军人才支持计划(国中医药人教发[2018]12号)
北京市自然科学基金资助项目(7204278)
北京中医药大学中青年教师项目(2019-JYB-JS-093)。
关键词
慢性脑缺血
活血化瘀
脑得生丸
自噬
神经元
AΒ42
大鼠
Chronic cerebral hypoperfusion
methods for activating blood circulation and removing blood stasis
Naodesheng Pills
autophagy
neuron
Aβ42
rats
