摘要
本研究探究了利用超高效液相色谱-串联质谱检测葡萄中28-表高芸苔素内酯残留的方法。葡萄样品采用QuPPe分析方法处理后,经超高效液相色谱柱分离,采用电喷雾负离子模式,通过多反应监测方式测定,基质匹配标准曲线外标法定量。结果表明,本方法线性相关系数>0.999;28-表高芸苔素内酯在添加水平为0.02~0.50 mg/kg条件下的回收率为78%~90%,相对标准偏差为4.4%~7.3%,其在葡萄样品上的定量限为0.02 mg/kg。本方法线性关系好,操作简单、准确、高效,适用于葡萄样品中28-表高芸苔素内酯农药残留的快速筛查和定量检测。
A method for the simultaneous identification of 28-homobrassinolide in grapes was developed using quick polar pesticides(QuPPe)extraction procedure combined with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).The analytes were separated by a UPLC column,detected in the negative ion mode(ESI-)using multiple reaction monitoring(MRM),and quantified by an external standard method.The result indicated that the correlation coefficient of this method was greater than 0.999.28-homobrassinolide's recovery range was 78%-90%when the adding level was 0.02-0.50 mg/kg.The relative standard deviation range was 4.4%-7.3%.The limit of quantitation of 28-homobrassinolide in grapes was 0.02 mg/kg.Results suggested that the method was suitable for rapid screening and quantitative analysis of 28-homobrassinolide in grapes with convenient,high precision and efficient,good linear relationship.
作者
杜功名
查欣欣
吴甜甜
陈刘阳
王洪雷
DU Gongming;ZHA Xinxin;WU Tiantian;CHEN Liuyang;WANG Honglei(Jianshu Evertest Limited Company,Nanjing210046,China;Jiangsu Pesticide Research Institute Co.,Ltd.,Nanjing 210046,China)
出处
《现代农药》
CAS
2021年第6期44-46,共3页
MODERN AGROCHEMICALS
