摘要
目的研究长片金线兰多糖的结构特征以及对四氯化碳(CCl_(4))致小鼠急性肝损伤的保护作用。方法从长片金线兰中提取多糖,分析其相对分子质量、单糖组成、紫外与红外吸收特征。将48只ICR小鼠随机分为对照组、模型组、联苯双酯(150 mg/kg)组和长片金线兰多糖低、中、高剂量(100、200、400 mg/kg)组,给予相应药物干预9 d后,除对照组外,其余各组ip CCl_(4)造成急性肝损伤,16 h后检测各组小鼠血清中丙氨酸转氨酶(alanine aminotransferase,ALT)、天冬氨酸转氨酶(aspartate aminotransferase,AST)和乳酸脱氢酶(lactate dehydrogenase,LDH)活性;测定各组大鼠肝脏组织中超氧化物歧化酶(superoxide dismutase,SOD)、过氧化氢酶(catalase,CAT)活性以及谷胱甘肽(glutathione,GSH)和丙二醛(malondialdehyde,MDA)含量;采用苏木素-伊红(HE)染色法观察各组小鼠肝脏组织病理变化。结果长片金线兰多糖的相对分子质量为4.254×105,由甘露糖、鼠李糖、半乳糖醛酸、葡萄糖、半乳糖、木糖和阿拉伯糖组成(物质的量比为1∶0.11∶0.19∶11.15∶0.87∶0.36∶0.18),紫外与红外光谱扫描结果推测其可能为一种吡喃型杂多糖。与模型组比较,长片金线兰多糖能够显著降低CCl_(4)致肝损伤小鼠血清中ALT、AST和LDH活性(P<0.05、0.01),提高肝脏组织中SOD、CAT活性和GSH含量(P<0.05、0.01),降低MDA含量(P<0.01),减轻CCl_(4)对肝组织的病理损伤。结论长片金线兰多糖对CCl_(4)致小鼠急性肝损伤具有一定的保护作用,其机制可能与抑制脂质过氧化有关。
Objective To study the structural characteristics of polysaccharides from Anoectochilus longilobus(ALP)against CCl;-induced acute liver injury in mice.Methods Polysaccharide was isolated from A.longilobus,its total carbohydrate content,relative molecular weight and monosaccharide composition were measured.Its structural characteristics were analyzed by ultraviolet(UV)and infrared(IR)spectroscopy.Forty-eight ICR mice were randomly divided into control group,model group,bifendate(150 mg/kg)group,low-,medium-,and high-dose(100,200,400 mg/kg)ALP groups.After 9 d of intervention with corresponding drugs,except control group,rats in other groups were ip CCl;to cause acute liver injury.After 16 h,AST,ALT and LDH activities in serum and SOD,CAT activities,GSH,MDA contents in liver of mice in each group were measured.Hematoxylin-eosin(HE)staining method was used to observe pathological changes in liver of mice in each group.Results Relative molecular mass of ALP was 4.254×10^(5),which was composed of mannose,rhamnose,galacturonic acid,glucose,galactose,xylose and arabinose with a molar ratio of 1:0.11:0.19:11.15:0.87:0.36:0.18.The results of UV and IR spectra showed that ALP may be a pyran-type heteropolysaccharide.Compared with model group,ALP significantly reduced ALT,AST and LDH activities in serum of mice with liver injury caused by CCl;(P<0.05,0.01),increased SOD,CAT activities and GSH content in liver tissue(P<0.05,0.01),reduced MDA content(P<0.01),and reduced the pathological damage of liver tissue caused by CCl;.Conclusion ALP has a certain protective effect on CCl;-induced acute liver injury in mice,and its mechanism may be related to the inhibition of lipid peroxidation.
作者
江颖倩
彭梦超
吴建国
吴岩斌
吴锦忠
JIANG Ying-qian;PENG Meng-chao;WU Jian-guo;WU Yan-bin;WU Jin-zhong(College of Pharmacy,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China)
出处
《中草药》
CAS
CSCD
北大核心
2021年第19期5932-5938,共7页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金资助项目(81773846)
国家自然科学基金资助项目(81603230)
福建省科技厅高校产学合作项目(2020Y4015)
福建省自然科学基金资助项目(2020J01735)。
关键词
长片金线兰
多糖
四氯化碳
急性肝损伤
保护作用
Anoectochilus longilobus H.Jiang et H.Z.Tian
polysaccharide
CCl4
acute liver injury
protective effect
