摘要
以胃蛋白酶和胰蛋白酶在体外模拟胃肠环境,测定模拟消化前后玉米低聚肽的相对分子质量分布、DPPH自由基清除能力、·OH清除能力、ABTS自由基清除能力、ORAC值和ROS清除能力,旨在探索模拟胃肠道消化对玉米低聚肽抗氧化活性的影响。结果表明:玉米低聚肽中大部分肽段相对分子质量低于1000。分别模拟胃、肠环境消化后,重均相对分子质量有所下降,下降率分别为1.47%、0.05%,ABTS自由基清除能力有一定程度的升高,分别为25.47%、1.39%。模拟胃环境消化前后,DPPH自由基清除能力IC_(50)值分别约为1.41、1.50 mg/mL,·OH清除能力IC_(50)值分别约为10.3、12.3 mg/mL;模拟肠环境消化前后,DPPH自由基清除能力IC_(50)值分别约为1.39、1.48 mg/mL,·OH清除能力IC_(50)值分别约为8.1、9.5 mg/mL。模拟胃环境消化后,ORAC值有所降低,降幅为10.4%,100、400μg/mL玉米低聚肽对ROS清除能力分别提高了1.42%、16.78%;模拟肠环境消化后,ORAC值提高了1.4%,100μg/mL玉米低聚肽对ROS清除能力降低1.71%,而400μg/mL玉米低聚肽对ROS清除能力提高2.61%。
The molecular weight distribution,DPPH radical scavenging ability,·OH scavenging ability,ABTS radical scavenging ability,ORAC value and ROS scavenging ability of corn oligopeptides(COPs)before and after simulated digestion by pepsin and trypsin in vitro were measured,in order to explore the effect of simulated gastrointestinal digestion on the antioxidant activity of COPs.The results showed that the relative molecular weight of most peptides in COPs was less than 1000 u.After simulated gastric and intestinal digestion,the weight average molecular weight decreased by 1.47%and 0.05%,respectively,and the free radical scavenging capacity of ABTS increased to a certain extent,25.47%and 1.39%,respectively.Before and after digestion in simulated gastric environment,the IC_(50) values of DPPH free radical scavenging capacity were about 1.41 and 1.50 mg/mL,and the IC_(50) values of·OH scavenging capacity were about 10.3 and 12.3 mg/mL,respectively.Before and after digestion in simulated intestinal environment,the IC_(50) values of DPPH free radical scavenging capacity were about 1.39 and 1.48 mg/mL respectively,and the IC_(50) values of·OH scavenging capacity were about 8.1 and 9.5 mg/mL,respectively.After simulated gastric digestion,ORAC value decreased by 10.4%,and ROS scavenging ability of 100μg/mL and 400μg/mL COPs increased by 1.42%and 16.78%,respectively.After simulated intestinal digestion,ORAC value increased by 1.4%,ROS scavenging activity of 100μg/mL COPs decreased by 1.71%,and ROS scavenging activity of 400μg/mL COPs increased by 2.61%.
作者
张江涛
张铭皓
高丽辉
冯晓文
谷瑞增
李国明
刘文颖
ZHANG Jiangtao;ZHANG Minghao;GAO Lihui;FENG Xiaowen;GU Ruizeng;LI Guoming;LIU Wenying(China National Research Institute of Food and Fermentation Industries Co.,Ltd./Beijing Engineering Research Center of Protein&Functional Peptides,Beijing 100015,China;College of Biological Sciences and Bio-Technology,Beijing Forestry University,Beijing 100083,China;College of Food Science and Engineering,Beijing University of Agriculture,Beijing 102206,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2021年第11期54-61,共8页
Journal of Food Science and Biotechnology
基金
国家“十三五”重点研发计划项目(2016YFD0400604)
国家自然科学基金项目(31671963)
北京市科技创新基地培育与发展工程专项(Z191100002819001)。
关键词
玉米低聚肽
体外消化
抗氧化
相对分子质量
人肝星状细胞
corn oligopeptides
in vitro digestion
antioxidant activity
relative molecular weight
human hepatic stellate cells(HSC)
