摘要
为明确玉米秸秆腐解产物对假禾谷镰刀菌的影响,采用生长速率法和涂布平板法分别测定玉米秸秆腐解液对假禾谷镰刀菌(Fusarium pseudograminearum)菌丝生长和孢子萌发的影响;采用DNS法测定腐解液对该菌多聚半乳糖醛酸酶(PG)和果胶甲基半乳糖醛酸酶(PMG)活性;采用Hoffman法测定多聚半乳糖反式消除酶(PGTE)和果胶甲基反式消除酶(PMTE)活性。结果表明:玉米秸秆腐解0 d、7 d和14 d对菌丝生长和孢子萌发均表现低促高抑的作用,浓度高于0.0625g·DW/mL腐解液处理均表现抑制作用,且随腐解天数的增加抑制作用增强,两者抑制率最高分别达100%和93.7%。腐解液对假禾谷镰刀菌PMTE和PGTE活性均表现抑制作用,抑制率分别在1.2%~99.0%和6.6%~99.6%;腐解7 d和14 d、浓度低于0.0625 g·DW/mL腐解液处理,PG活性在8.787~10.420 U/mg,与对照相比增幅在4.2%~22.2%;PMG活性在7.557~8.177 U/mg,与对照相比增幅在2.3%~10.7%。该研究揭示了秸秆还田条件下小麦茎基腐病重发的原因。
In order to clarify the effects of decomposed maize straw liquid on F.pseudograminearum,the growth rate method and coating plate method were used to determine the effect of corn straw decomposition solution on mycelial growth and spore germination of F.pseudograminearum.The activities of PG and PMG were determined by DNS method,and the activities of PGTE and PMTE were determined by Hoffman method.The results showed that 0,7 and 14 days after decomposition of corn straw had low promoting and high inhibiting effects on mycelial growth and spore germination,and the treatments with the concentration higher than 0.0625 g·DW/mL had inhibitory effects,and the inhibitory effect became stronger with the increase of decomposition days,with the highest inhibition rates of 100%and 93.7%,respectively.The decomposed maize straw liquid showed an inhibition on activities of PMTE and PGTE of F.pseudograminearum,and the inhibition rates were between 1.2%to 99.0%and 6.6%to 99.6%,respectively.The results showed that PG activity increased by 4.2%~22.2%and PMG activity increased by 2.3%~10.7%in the range of 8.787~10.420 U/mg and 7.557~8.177 U/mg,respectively,compared with the control,when the decomposition solution concentration was lower than 0.0625 g·DW/mL for 7 and 14 days.This study reveals the causes of repeated occurrence of wheat crown rot under the condition of straw returning to field.
作者
马璐璐
闫翠梅
王芳芳
王丽
张天雅
齐永志
甄文超
MA Lulu;YAN Cuimei;WANG Fangfang;WANG Li;ZHANG Tianya;QI Yongzhi;ZHEN Wenchao(College of Plant Protection,Hebei Agricultural University,Baoding 071001;College of Agronomy,Hebei Agri-cultural University/Hebei Key Laboratory of Crop Growth Regulationf,Baoding 071001,China)
出处
《东北农业科学》
2021年第5期51-55,共5页
Journal of Northeast Agricultural Sciences
基金
“十三五”国家重点研发计划(2017YFD0300906)
河北省自然科学基金(C2016204211)
河北省教育厅项目(ZD2016162)。
关键词
玉米秸秆腐解液
假禾谷镰刀菌
菌丝生长
孢子萌发
细胞壁降解酶
Decomposed corn straw liquid
Fusarium pseudograminearum
Mycelial growth
Spore germination
Cell wall degradation enzyme
