摘要
以广西崇左余甘子果核为原材料,通过单因素试验和正交试验设计优化超声波辅助提取余甘子果核多酚工艺条件,并以对羟基自由基(·OH)及1,1-二苯基-2-三硝基苯肼自由基(DPPH·)的清除效果评价余甘子果核多酚的抗氧化活性。结果表明,余甘子果核多酚的最佳提取工艺条件为使用体积分数70%丙酮、料液比1∶40(g∶mL)、提取温度40℃及提取时间30 min。在此优化条件下,余甘子果核多酚得率为7.37%。余甘子果核多酚对·OH和DPPH·的清除效果与浓度间存在正相关的量效关系,当余甘子果核多酚质量浓度为0.05 mg/mL时,对DPPH·的清除率为80.53%;当余甘子果核多酚质量浓度为3.0 mg/mL时,对·OH的清除率达84.44%,半抑制浓度(IC50)值为1.2 mg/mL。结果表明余甘子果核多酚具有一定的抗氧化活性。
Taking Phyllanthus emblica L. kernel from Chongzuo, Guangxi as raw materials, the ultrasound-assisted extraction process of polyphenols from P. emblica L. kernel was optimized by single factor test and orthogonal experiments, and the antioxidant activity of polyphenols from P. emblica L. kernel was evaluated by scavenging effects on hydroxyl free radical(·OH) and 1,1-diphenyl-2-picrylhydrazyl free radical(DPPH·). The results showed that the optimum extraction technology conditions were obtained as follows: acetone volume fraction 70%, solid and liquid ratio 1∶40(g∶ml), extraction temperature40 ℃, and time 30 min. Under these optimal conditions, the extraction rate of polyphenols from P. emblica L. kernel was 7.37%. The scavenging effects of the polyphenols on ·OH and DPPH· showed positive dose-effect relationship with the polyphenols concentration. When the mass concentration of the polyphenols was 0.05 mg/ml, the scavenging rate of DPPH·was 80.53%. When the mass concentration of the polyphenols was 3.0 mg/ml, the scavenging rate of·OH reached 84.44%, and half inhibitory concentration(IC50) value was 1.2 mg/ml. The results showed that the polyphenols from P. emblica L. kernel had certain antioxidant activity.
作者
黄秋萍
黄秋婵
郑燕菲
韦友欢
翁艳英
黄晓敏
曾振芳
HUANG Qiuping;HUANG Qiuchan;ZHENG Yanfei;WEI Youhuan;WENG Yanying;HUANG Xiaomin;ZENG Zhenfang(Guangxi College and Universities Key Laboratory Breeding Base of Chemistry of Guangxi Southwest Plant Resources,College of Chemical and Biological Engineering,Guangxi Normal University for Nationalities,Chongzuo 532200,China)
出处
《中国酿造》
CAS
北大核心
2021年第10期210-214,共5页
China Brewing
基金
广西民族师范学院科研项目(2018YB019、2020YB011)
广西民族师范学院国家基金培育项目(2020GP008)
广西民族师范学院硕士点学科建设项目(327003070416)
广西高校中青年教师基础能力提升项目(2017KY0846、2021KY0773)。
关键词
余甘子果核
多酚
超声辅助
提取工艺
抗氧化活性
Phyllanthus emblica L.kernel
polyphenol
ultrasound-assisted
extraction technology
antioxidant activity
