摘要
目的探讨血清长链非编码RNA小核仁RNA宿主基因16(lncRNA SNHG16)联合微小RNA(miR)-16-5p检测对胃癌的诊断价值。方法选取武汉市第三医院2018年1月至2020年1月收治的102例胃癌患者作为胃癌组,另选取同期102名体检健康者为对照组。对比2组血清lncRNA SNHG16、miR-16-5p水平,分析血清lncRNA SNHG16、miR-16-5p水平与胃癌患者临床及病理参数的关系,Pearson相关性方法分析血清lncRNA SNHG16与miR-16-5p水平的相关性,受试者工作特征(ROC)曲线分析血清lncRNA SNHG16、miR-16-5p水平对胃癌的诊断价值。结果胃癌组血清lncRNA SNHG16水平明显高于对照组,miR-16-5p水平明显低于对照组[(2.03±0.17)比(1.69±0.34)、(0.56±0.06)比(0.82±0.28)](均P <0.001)。胃癌患者血清lncRNA SNHG16、miR-16-5p水平与TNM分期、侵袭程度、淋巴结转移、远处转移相关(均P <0.05)。Pearson相关性分析结果显示,胃癌患者血清lncRNA SNHG16与miR-16-5p水平呈负相关(r=-0.405,P <0.001)。ROC曲线分析显示,lncRNA SNHG16与miR-16-5p联合[曲线下面积(AUC)=0.903,95%置信区间0.854~0.940]诊断胃癌的AUC大于lncRNA SNHG16(AUC=0.816,95%置信区间0.755~0.866)、miR-16-5p(AUC=0.826,95%置信区间0.766~0.875)单独检测。结论胃癌患者血清lncRNA SNHG16水平显著提升,miR-16-5p水平显著降低,二者呈负相关,联合检测血清lncRNA SNHG16和miR-16-5p水平可提升胃癌诊断价值。
Objective To explore the diagnostic value of serum long non-coding RNA small nucleolar RNA host gene 16( lncRNA SNHG16) combined with microRNA( miR)-16-5 p detection for gastric cancer.Methods Totally 102 patients with gastric cancer admitted to Wuhan Third Hospital from January 2018 to January2020 was selected as gastric cancer group,and 102 healthy persons undergoing physical examination during the same period were selected as the control group. The serum lncRNA SNHG16 and miR-16-5 p levels were compared between the two groups,and the relationship between the serum lncRNA SNHG16,miR-16-5 p levels and the clinical and pathological parameters of gastric cancer patients were analyzed. The correlation between the serum lncRNA SNHG16 and miR-16-5 p levels was analyzed by Pearson correlation. Receiver operating characteristic( ROC) curve were used to analyzed the value of serum lncRNA SNHG16 and miR-16-5 p levels in the diagnosis of gastric cancer. Results The level of serum lncRNA SNHG16 in gastric cancer group was significantly higher than that in control group,and the level of miR-16-5 p in gastric cancer group was significantly lower than that in control group[( 2. 03 ± 0. 17) vs( 1. 69 ± 0. 34),( 0. 56 ± 0. 06) vs( 0. 82 ± 0. 28) ]( both P < 0. 001). The serum levels of lncRNA SNHG16 and miR-16-5 p in patients with gastric cancer were correlated with TNM stage,degree of invasion,lymph node metastasis,and distant metastasis( all P < 0. 05). Pearson correlation analysis showed that serum lncRNA SNHG16 were negatively correlated with miR-16-5 p level in patients with gastric cancer( r =-0. 405,P < 0. 001). The ROC curve showed that the area under the curve( AUC) of lncRNA SNHG16 + miR-16-5 p detection[AUC =0. 903,95% confidence interval( CI) : 0. 854-0. 940]in the diagnosis of gastric cancer was higher than that of lncRNA SNHG16( AUC = 0. 816,95% CI: 0. 755-0. 866) and miR-16-5 p( AUC = 0. 826,95% CI: 0. 766-0. 875)alone. Conclusions The level of serum lncRNA SNHG16 in patients with gastric cancer significantly increases,and the level of miR-16-5 p significantly reduces. The two indexes are negatively correlated. Combined detection of serum lncRNA SNHG16 and miR-16-5 p levels can improve the diagnostic value in gastric cancer.
作者
杨季
彭波
李军
陈雯雯
李周彤昕
Yang Ji;Peng Bo;Li Jun;Chen Wenwen;Li Zhoutongxin(Department of Clinical Laboratory,Wuhan Third Hospital,Wuhan 430000,China;Department of Gastroenterology,Wuhan Third Hospital,Wuhan 430000,China)
出处
《中国医药》
2021年第10期1475-1479,共5页
China Medicine
基金
湖北省卫生和计划生育委员会科研项目(WJ2017F021)。
