摘要
目的探讨华蟾素对肾癌细胞侵袭和迁移的影响,并探讨其对Wnt/β-catenin信号通路的调控机制。方法将肾癌细胞分为对照组(添加PBS)和华蟾素组(添加华蟾素),CCK-8法检测细胞增殖力,Transwell侵袭和细胞迁移法检测细胞侵袭和迁移力,流式细胞检测细胞凋亡和细胞周期,免疫蛋白印迹法检测Wnt/β-catenin和上皮间质变(epithelial mesenchymal transition,EMT)信号通路相关蛋白表达。结果与对照组相比,华蟾素组可抑制肾癌细胞增殖,抑制细胞侵袭和迁移力,促进细胞凋亡,阻滞细胞周期(P<0.05);E-cadherin蛋白表达上调(P<0.05),Wnt/β-catenin、N-cadherin、Vimentin蛋白表达下调(P<0.05)。结论华蟾素可通过调控Wnt/β-catenin信号通路,逆转EMT,抑制肾癌细胞增殖和侵袭。
Objective To investigate the effect of bufalin on the invasion and migration of renal carcinoma cells,and to explore its regulation mechanism of Wnt/β-catenin signalling pathway.Methods The ability of cell proliferation were detected with CCK-8 kits.Transwell and cell wound scratch assays were applied to evaluate the ability of cell invasion and migration.The cell apoptosis and cell cycle were detected by flow cytometry.Western blotting was used to detect the proteins expression of Wnt/β-catenin signalling pathway and epithelial mesenchymal transition(EMT).Results Compared with control group,bufalin group inhibited the cell proliferation,suppressed cell invasion and migration,promoted cell apoptosis,and blocked cell cycle in the renal cell carcinoma(P<0.05).The protein expression of E-cadherin was up-regulated,and Wnt,β-catenin,N-cadherin and Vimentin was down-regulated(P<0.05).Conclusion Bufalin can inverse EMT and inhibit proliferation and invasion of renal cancer cells by regulating the Wnt/β-catenin signalling pathway.
作者
吴潇芸
郑盛锋
张明津
付伟金
WU Xiao-yun;ZHENG Sheng-feng;ZHANG Ming-jin;FU Wei-jin(Guangxi Medical College,Nanning 530023,Guangxi,China)
出处
《广东医学》
CAS
2021年第10期1172-1176,共5页
Guangdong Medical Journal
基金
广西医疗卫生适宜技术开发与推广应用项目(S2017034)
广西卫健委2021年西医自筹课题(Z20211284)。
