摘要
目的分析骆驼刺中分离富集的紫铆素对人宫颈癌SiHa细胞增殖和凋亡的影响及机制。方法以CCK-8法检测紫铆素对SiHa细胞增殖的抑制作用,流式细胞术检测紫铆素对细胞凋亡水平和细胞周期影响,RT-PCR法检测紫铆素对细胞凋亡相关基因表达的影响,Western Blot法检测凋亡相关蛋白的表达。结果与空白对照组比较,紫铆素可明显诱导SiHa细胞发生凋亡(P<0.01),紫铆素干预SiHa细胞可使克隆形成数量明显减少(P<0.05),细胞凋亡率明显升高(P<0.05,P<0.01),线粒体膜电位明显下降(P<0.01),G0/G1期细胞所占的百分比增加(P<0.01),胱天蛋白酶3(Caspase-3)和胱天蛋白酶7(Caspase-7)的基因和蛋白表达增加(P<0.05,P<0.01),同时Caspase-3的底物PARP蛋白被剪切活化(P<0.05),亦明显降低Bcl-2的基因和蛋白表达(P<0.05,P<0.01)。结论紫铆素对SiHa细胞的增殖具有抑制作用,其抑制作用可能与促进细胞凋亡有关。
Objective To analyze the effect and mechanism of butin(BUN)from Alhagi sparsifolia on proliferation and apoptosis of human cervical cancer SiHa cells.Methods The CCK-8 method was used to detect the inhibitory effect of BUN on the proliferation of human cervical cancer SiHa cells,the apoptosis level and cell cycle of cervical cancer cells were detected by flow cytometry.The effect of BUN on apoptosis-related mRNA of SiHa cells was detected by RT-PCR;Western Blot was used to detect the expression of apoptosis-related proteins.Results Compared with the control group,BUN could significantly reduce apoptosis of SiHa cells(P<0.01),the number of clones was significantly decreased(P<0.05),and the apoptosis rate was significantly increased(P<0.05,P<0.01).The mitochondrial membrane potential decreased significantly(P<0.01),the percentage of G0/G1 phase cells increased(P<0.01),the expression of Caspase 3 and Caspase 7 increased(P<0.05,P<0.01),the substrate of Caspase-3(PARP)was activated(P<0.05),and the expression of Bcl-2 was significantly decreased(P<0.05,P<0.01).Conclusion BUN has an inhibitory effect on the proliferation of cervical cancer SiHa cells,and its inhibitory effect may be related to the promotion of cell apoptosis.
作者
马晓玲
刘雪松
石磊岭
陈刚
李宁
魏鸿雁
MA Xiaoling;LIU Xuesong;SHI Leiling;CHEN Gang;LI Ning;WEI Hongyan(Xinjiang Institute of Chinese Medicine and National Medicine,Urumqi 830002 Xinjiang,China;College of Life Sciences and Technology,Xinjiang University,Urumqi 830046 Xinjiang,China;School of Traditional Chinese Medicine,Shenyang Pharmaceutical University,Shenyang 110016 Liaoning,China)
出处
《中药新药与临床药理》
CAS
CSCD
北大核心
2021年第9期1284-1292,共9页
Traditional Chinese Drug Research and Clinical Pharmacology
基金
乌鲁木齐市天山区科学技术计划项目(2018006)。
关键词
骆驼刺
紫铆素
SIHA细胞
增殖
凋亡
Alhagi sparsifolia shap.ex Keller&Shap.
butin
SiHa cells
proliferation
apoptosis
