摘要
选择20批次枳实,以肉和瓤2个部位为研究对象。采用UPLC分别建立枳实肉和瓤的指纹图谱,肉中标定17个共有峰,瓤中标定10个共有峰,指认了6种黄酮类成分,分别为芸香柚皮苷、柚皮苷、野漆树苷、橙皮苷、新橙皮苷和川陈皮素。采用《中药色谱指纹图谱相似度评价系统》软件进行相似度分析,枳实肉和瓤与各自的对照图谱相似度较好,均大于0.90;同一批次枳实肉和瓤的相似度为0.850~0.983。采用SPSS 17.0和SIMCA 14.1软件对枳实肉和瓤的共有峰进行聚类分析、主成分分析和正交偏最小二乘法判别分析,结合对照图谱共确定枳实肉和瓤的12个差异性成分。在此基础上对6种指认的黄酮类成分和辛弗林进行含量测定。所建立的UPLC指纹图谱和多成分定量分析方法可用于枳实的质量评价,研究结果为枳实"翻肚如盆口"性状特征的科学内涵提供部分科学依据。
Twenty batches of Aurantii Fructus Immaturus(AFI)were collected,with their peel and pulp taken as research objects.Ultra-high performance liquid chromatography(UPLC)fingerprints of peel and pulp of AFI were established with 17 common peaks in peel and 10 in pulp.Six kinds of flavonoids were identified,i.e.,narirutin,naringin,rhoifolin,hesperidin,neohesperidin and nobiletin.The Similarity Evaluation System for Chromatographic Fingerprint of Traditional Chinese Medicine was employed for similarity analysis,which showed that the chromatographic peaks of peel and pulp were basically similar to their respective reference fingerprints,with all similarities greater than 0.90.The similarity between peel and pulp of the same batch of AFI ranged from 0.850 to 0.983.Cluster analysis(CA),principal component analysis(PCA),and orthogonal partial least squares discriminant analysis(OPLS-DA)were conducted on the common peaks of peel and pulp of AFI with SPSS 17.0 and SIMCA 14.1.Combined with the reference fingerprints,these analyses revealed 12 differential components regarding peel and pulp.Further,the content of the 6 flavonoids and synephrine was determined.The proposed method integrating UPLC fingerprint and multicomponent quantitative analysis is applicable to the quality evaluation of AFI.The results provide a certain basis for the scientific connotation about the appearance characteristic of AFI.
作者
石敬依
蔡文君
林文栋
张硕
罗容
SHI Jing-yi;CAI Wen-jun;LIN Wen-dong;ZHANG Shuo;LUO Rong(School of Traditional Chinese Medicine,Capital Medical University,Beijing 100069,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2021年第17期4446-4455,共10页
China Journal of Chinese Materia Medica
基金
国家自然科学基金面上项目(81973431)。
关键词
枳实
UPLC指纹图谱
性状特征
化学模式识别
黄酮
辛弗林
Aurantii Fructus Immaturus
UPLC fingerprint
characteristics
chemical pattern recognition
flavonoid
synephrine
