摘要
以黄芩苷得率为指标,采用响应面法考察对黄芩苷得率影响较大的乙醇浓度、液料比和提取时间三个因素的最优区域,从而筛选出黄芩苷醇提工艺的最优条件。通过实验得到最佳醇提工艺为黄芩苷在液料比为10:1的77%的乙醇溶剂中加热回流2 h,提取2次,该条件下黄芩苷得率达12.55%。结果表明响应面法优化黄芩苷醇提工艺易行可控,能够得到较高含量的黄芩苷。
Taking the yield of baicalin as the index,response surface methodology was used to investigate the three factors which had great influence on the yield of baicalin:ethanol concentration,ratio of liquid to material and extraction time.The optimal conditions of ethanol extraction of baicalin were obtained.The results showed that the best ethanol extraction process was that baicalin was heated and refluxed for 2 h in 77%ethanol solvent with a ratio of liquid to material of 10:1,and the extraction was twice.Under this condition,the yield of baicalin was 12.55%.The results showed thatthe optimization of ethanol extraction process of baicalin by response surface methodology was easy and controllable,and higher content of baicalin could be obtained.
作者
金尧
王佰灵
李欧
JIN Yao;WANG Bai-ling;LI Ou(College of Pharmacy,Hubei University of Medicine,Hubei Shiyan 442000;Hubei Key Laboratory of Wudang Local Chinese Medicine Research,Hubei Shiyan 442000,China)
出处
《广州化工》
CAS
2021年第18期54-57,共4页
GuangZhou Chemical Industry
基金
湖北省十堰市2019年一般项目(19K68)
湖北省科技厅中央引导地方科技发展专项(2019ZYYD071)。
关键词
黄芩
黄芩苷
醇提工艺
星点设计
Scutellariabaicalensis
baicalin
ethanol extraction process
central composite design-response surface methodology
