摘要
采用醇提法提取核桃青皮有效成分,用乙酸乙酯、正丁醇萃取得到不同萃取物。SPF级雄性SD大鼠随机分为正常对照组、模型组、阳性对照组、核桃青皮乙醇提取物组、乙酸乙酯萃取物组、正丁醇萃取物组,给予高脂饲料12周,灌胃给药6周后,检测大鼠血清中总胆固醇(total cholesterol,TC)、甘油三酯(triglyceride,TG)、高密度脂蛋白胆固醇(high density lipoprotein cholesterol,HDL-C)、低密度脂蛋白胆固醇(low density lipoprotein cholesterol,LDL-C)、谷氨酸氨基转移酶(glutamate aminotransferase,ALT)、天冬氨酸氨基转移酶(aspartate aminotransferase,AST);肝组织中丙二醛(malondialdehyde,MDA)含量,超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-Px)活性;用苏木精-伊红染色法(hematoxylin-eosin staining,HE)进行肝组织病理学切片分析。结果表明,与正常组相比,模型组大鼠TG、TC、HDL-C、ALT、AST和MDA含量显著升高(P<0.01),LDL-C含量和GSH-Px、SOD活性显著降低(P<0.001),肝组织病理损伤明显;与模型组相比,核桃青皮乙醇提取物组、乙酸乙酯萃取物组、正丁醇萃取物组的TG、TC、HDL-C、ALT、AST、MDA含量显著降低(P<0.05),LDL-C含量和GSH-Px、SOD活性显著升高(P<0.01);肝组织病理损伤有明显改善;其中,正丁醇萃取物效果更好。
The effective components of walnut green husk were extracted by alcohol extraction,and different extracts were obtained by extraction with ethyl acetate and n-butanol.The effect of different extracts from walnut green husk on non-alcoholic fatty liver disease(NAFLD)were investigated.Specific pathogen free(SPF)male Sprague Dawley(SD)rats were randomly divided into normal control group,model group,positive control group and walnut green husk ethanol,ethyl acetate and n-butanol group.The rats were fed with high-fat diet for 12 weeks and given intragastric administration for 6 weeks.The serum levels of total cholesterol(TC),triglyceride(TG),high-density lipoprotein cholesterol(HDL-C),low-density lipoprotein cholesterol(LDL-C),glutamate aminotransferase(ALT)and aspartate aminotransferase(AST)were measured.The content of malondialdehyde(MDA),the activities of superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)in liver tissue were checked.Pathological sections were analyzed by hematoxylin-eosin staining(HE).Compared with the normal group,the contents of TG,TC,HDL-C,ALT,AST and MDA in the model group were significantly increased(P<0.01),while the contents of LDL-C,GSH-Px and SOD activities were significantly decreased(P<0.001),and the pathological damage of liver tissue was obvious.Compared with the model group,the contents of TG,TC,HDL-C,ALT,AST and MDA in the ethanol,ethyl acetate and n-butanol extracts of walnut green husk decreased significantly(P<0.05),while the contents of LDL-C and the activities of GSH-Px and SOD increased significantly(P<0.01).Pathological damage was improved obviously.The n-butanol extract had more obvious effect than other extracts.
作者
方琼莲
王蒙蒙
乔雪
李欣坪
黄丰
林玉萍
赵声兰
FANG Qionglian;WANG Mengmeng;QIAO Xue;LI Xinping;HUANG Feng;LIN Yuping;ZHAO Shenglan(School of Chinese Materia Medica and Yunnan Key Laboratory of Southern Medicinal Resource,Yunnan University of Chinese Medicine,Kunming 650500,China)
出处
《食品与发酵工业》
CAS
CSCD
北大核心
2021年第18期159-164,共6页
Food and Fermentation Industries
基金
云南省科技厅中医联合专项(2017FF117-022,2017FF116-016)
生物资源数字化开发应用项目(202002AA100007)。
