摘要
目的:优化红豆树叶总黄酮提取工艺并研究其抗氧化性。方法:采用超声辅助法分别考察液料比、乙醇浓度和超声时间对红豆树叶总黄酮提取率的影响。以单因素试验为依据,进行响应面设计试验及数据分析,进而对其工艺进行优化设计,同时进行总黄酮的抗氧化性试验。结果:各因素对提取率的影响由大到小顺序为乙醇浓度、液料比和超声时间;且最佳提取工艺为乙醇浓度69.45%,液料比20.15∶1(mL/g),超声时间90.17 min。在此工艺下,总黄酮提取率为23.85%,通过验证试验发现,试验提取率为23.87%,与预测值相对标准偏差为0.06%;同时红豆树叶总黄酮对DPPH自由基和ABTS自由基表现出较好的清除效果。结论:优化后的红豆树叶总黄酮提取工艺稳定可行,并且其具有较强的抗氧化能力。
Objective:Optimization of the extraction technology of total flavonoids from leaves of Ormosia hosiei and study on its antioxidant activity.Methods:The effects of liquid to solid ratio,ethanol concen⁃tration and ultrasonic time on the extraction rates of total flavonoids from leaves of O.hosiei were investi⁃gated by ultrasonic-assisted extraction.Based on the results of single-factor test,response surface design and data analysis were carried out to further optimize the ultrasonic-assisted extraction.At the same time,the antioxidant activity of total flavonoids was tested.Results:The influence of factors on flavo⁃noids extraction rate was in turn ethanol concentration,liquid to solid ratio,the ultrasonic time.The opti⁃mal extraction process conditions were liquid to solid ratio20.15∶1(mL/g),ethanol concentration 69.45%and ultrasonic time 90.17 min.Under the above conditions,the extraction of total flavonoids was 23.85%.The experimental results showed that the extraction of total flavonoids was 23.87%,which was close to the predicted value and the relative standard deviation between the two was 0.06%.Meanwhile,finding the total flavonoids from leaves of O.hosiei had good scavenging effects on DPPH free radicals and ABTS free radicals.Conclusion:The optimized extraction process of total flavonoids from the leaves of O.hosiei was stable and workable,and it has a strong antioxidant ability.
作者
全颖萱
黄冰冰
贝佳炎
郑威
李林海
倪林
徐会有
Quan Yingxuan;Huang Bingbing;Bei Jiayan;Zheng Wei;Li Linhai;Ni Lin;Xu Huiyou(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Conservation and Utilization of Natural Biological Resources Fujian University Engineering Research Center,Fuzhou 350002,China.)
出处
《中国野生植物资源》
CSCD
2021年第8期20-26,共7页
Chinese Wild Plant Resources
基金
国家级大学生创新创业训练计划项目(202010389024)
国家级大学生创新创业训练计划项目(202110389002)
国家级大学生创新创业训练计划项目(202110389039X)
福建省本科教育教学改革项目(FBJG20180211)
制药工程学科竞赛指导团队(111416040)。
关键词
红豆树叶
总黄酮
超声辅助提取
响应面法
抗氧化性
Leaves of Ormosia hosiei
Total flavonoids
Uultrasonic assisted extraction
Response sur⁃face methodology
Antioxidant activity
