摘要
目的:优化龙牙楤木多糖复合酶法提取工艺,提高其抗氧化活性。方法:以纤维素酶、果胶酶和木瓜蛋白酶为复合酶提取龙牙楤木多糖,以酶解温度、酶解时间、料液比为自变量,通过Box-Behnken中心组合法设计三因素三水平试验方案,并通过测定自由基清除能力考察龙牙楤木多糖的抗氧化活性。结果:复合酶法提取龙牙楤木多糖的最佳工艺条件为:酶解温度54℃,酶解时间160 min,料液比(m龙牙楤木粗粉∶V纯化水)1∶31(g/mL),此条件下龙牙楤木多糖平均提取率为(10.68±0.05)%,与模型预测值10.78%接近;抗氧化试验表明龙牙楤木多糖对DPPH自由基及羟基自由基的IC 50值分别为2.00,3.05 mg/mL,且清除作用均随多糖质量浓度的增大而升高,清除率最大值分别为78.10%,70.10%。结论:该复合酶法工艺条件提高了龙牙楤木多糖提取率;龙牙楤木多糖具有良好的抗氧化活性。
Objective:This study aimed to optimization of polysaccharide extraction process from Aralia elata(Miq.)Seem by complex enzymes and research of its antioxidant activity.Methods:The cellulase,pectinase and papain were combined as the compound enzymes and used to extract the polysaccharide from A.elata(AEPS).Enzymolysis temperature,enzymolysis duration,and solid-liquid ratio as the independent variables,a three-level-three-factor scheme was designed using the Box-Behnken method.After that,the antioxidant activity of AEPS was determined by the free radical scavenging ability experiments.Results:The optimal process conditions for the extraction process of AEPS using complex enzymes were as follows:enzymolysis at 54℃for 160 min,with a ratio of solid-liquid 1∶31(g/mL).Under the control of these conditions,the average AEPS extraction rate was(10.68±0.05)%,closing to 10.78%predicted by the model.According to the DPPH assay,the half-maximal inhibitory concentrations(IC 50)of AEPS concerning the free radicals and hydroxyl radicals were 2.00 mg/mL and 3.05 mg/mL,respectively.With the increasing polysaccharide concentration,the scavenging rate increased,with the maximum scavenging of 78.10%and 70.10%.Conclusion:The extraction rate of polysaccharides of Aralia elata was improved by the compound enzyme method,and AEPS had higher antioxidant activity.
作者
张桂娟
陈继凌
陶荟竹
ZHANG Gui-juan;CHEN Ji-ling;TAO Hui-zhu(Heilongjiang Vocational Institute of Ecological Engineering,Haerbin,Heilongjiang 150025,China;Jinghua Pharmaceutical Group Co.,Ltd.,Nantong,Jiangsu 226000,China)
出处
《食品与机械》
北大核心
2021年第8期185-192,共8页
Food and Machinery
基金
黑龙江省森林工业总局科技计划项目(编号:sgzjY2013012)。
关键词
龙牙楤木
多糖
复合酶
抗氧化活性
Aralia elata Seem
polysaccharide
complex enzyme
antioxidant activity
