摘要
选用DNASTAR、ProtParam、SignalP5.0、MEGA等生物信息学平台,分析人源肝脏细胞色素代谢酶CYP3A4基因的序列、理化性质、结构域及信号通路等,对CYP3A4基因的结构与功能进行鉴定。结果表明:CYP3A4基因编码区长为1509碱基对(bp),共编码502个氨基酸(aa);CYP3A4蛋白分子式为C_(2620)H_(4123)N_(659)O_(723)S_(26),相对分子质量为57256.10,体外不稳定;CYP3A4二级结构以α螺旋和无规则卷曲为主,存在2个跨膜结构,信号肽位于第28~29 bp处,另有47个磷酸化位点和2个N-糖基化位点,存在20个B细胞抗原表位;基因CYP3A4在化合物联苯分解、黄酮类代谢、葡萄糖醛酸基转移酶活性的负调控以及视黄醇的新陈代谢和胆汁分泌等系列信号通路中发挥重要作用,是药物氧化代谢的核心体系;黑猩猩与人源CYP3A4蛋白相似性最高。
DNASTAR,ProtParam,SignalP5.0 and MEGA platforms were used to analyze the sequence,physical and chemical properties,domain and signal pathway of CYP3A4 gene of human cytochrome by bioinformatics,the structure and function of CYP3A4 gene protein were identified.The results show that the coding area of the gene is 1509 base pair(bp),and 502 amino acids(aa)are encoded.The molecular formula of the protein is C_(2620)H_(4123)N_(659)O_(723)S_(26),and the relative molecular weight of the protein is 57256.10,and the protein is unstable in vitro.The secondary structures of the protein are presence inα-spiral and irregular curl,and there are two transmembrane structure domains,and signal peptide is located at 28-29 bp,there are 47 phosphorylation sites,2 N-glycosylation sites,and 20 B cell epitopes.The CYP3A4 plays an important role in a series of signa pathways,such as biphenyl decomposition,flavonoid metabolism,glucuronidase activity,retinolmetabolism and bile secretion,in addition,and it is the core system of drug oxidation metabolism;Pan troglodytes has the highest similarity with human CYP3A4 protein.
作者
杨晴
冯承涛
YANG Qing;FENG Chengtao(School of Chemical Engineering,Anhui University of Science and Technology,Huainan 233100,China)
出处
《安徽工业大学学报（自然科学版）》
CAS
2021年第3期262-267,共6页
Journal of Anhui University of Technology（Natural Science）
基金
安徽省自然科学基金项目(2008085MB50)。
