摘要
目的基于网络药理学方法探讨姜黄-白芍药对治疗类风湿关节炎(RA)的协同机制。方法通过TCMSP平台和文献资料筛选姜黄-白芍药活性成分及对应的靶点蛋白,应用Cytoscape 3.7.1软件构建药物-成分-靶点网络。在GeneCards、OMIM、CTD数据库中检索“Rheumatoid Arthritis”,获取RA疾病靶点。对药对和RA共同靶点进行PPI网络分析,GO富集和KEGG通路富集分析。结果获取药对13种活性成分及445个潜在靶点,RA疾病2060个相关靶点,得到姜黄、白芍、RA三者共同靶点53个,PPI网络中根据自由度(degree)靠前的3个靶点基因为AKT1、TNF、PTGS2,GO富集结果为225个,姜黄与RA相关通路28条,白芍与相关通路74条,其中24条信号通路重合。结论姜黄-白芍药对可能通过调节AKT1、TNF、PTGS2等靶点,发挥抗氧化、抑制炎症反应、调节免疫功能及调控细胞凋亡等功能协同防治RA。
Objective To probe into the synergistic mechanism of Curcumaelongae Rhizoma and Paeoniae Radix Alba in the treatment of rheumatoid arthritis(RA)by network pharmacology.Methods The active components of the drug pair and predicted targets were collected from TCMSP public database and literature.The“drug-component-target”network was constructed by Cytoscape 3.7.1 software.Disease targets were obtained by searching“Rheumatoid Arthritis”in GeneCards,OMIM and CTD databases.The PPI network was built in STRING database,and the GO function annotation and KEGG signal pathway enrichment analysis were performed by ClueGo plugin.The drug-component-target-disease network of the pair was constructed,and the network feature analysis was made by network analyzer of the Cytoscape 3.7.1 software.Results Totally 13 components and 445 potential targets in the pair and 2060 targets relating to RA were excavated,and 53 common targets of the pair were anti-RA targets.The top three targets were AKT1,TNF and PTGS2 in the PPI network by degree.The GO function annotation was 225.KEGG pathways of anti-RA target from Curcumaelongae Rhizoma was 28,while Paeoniae Radix Alba was 74 which they shared 24 pathways in conmmon.Conclusion Curcumaelongae Rhizoma and Paeoniae Radix Alba may regulate AKT1,TNF or PTGS2 so as to antioxidise,inhibit the inflammatory response,regulate immune function and adjust apoptosis to treat rheumatoid arthritis synergistically.
作者
凌益
徐晖
黄颖
任妮娜
唐芳
姚血明
陆道敏
马武开
Ling Yi;Xu Hui;Huang Ying(Clinical Application of Integrated Traditional Chinese and Western Medicine in Postgraduate College of Guizhou University of Traditional Chinese Medicine, Guiyang 550001;The Second Affiliated Hospital of Guizhou University of Traditional Chinese Medicine, Guiyang 550001)
出处
《安徽医科大学学报》
CAS
北大核心
2021年第7期1094-1100,共7页
Acta Universitatis Medicinalis Anhui
基金
国家自然科学基金(编号:81760907、81760829)
贵州省高层次创新型人才培养计划—“百”层次人才项目[编号:黔科合平台人才(2016)5650]
贵州省风湿免疫病临床研究中心[编号:黔科合平台人才(2020)2202]。
关键词
类风湿关节炎
姜黄-白芍
网络药理学
rheumatoid arthritis
Curcumaelongae Rhizoma and Paeoniae Radix Alba
network pharmacology
