摘要
RNAi作为一种基因沉默的分子生物学技术,广泛应用于生物基因功能研究。在昆虫RNAi研究中,病毒诱导的基因沉默(Virus-induced gene silencing,VIGS)技术是一种将dsRNA导入昆虫体内的独特策略。目前,这种方法在半翅目和鳞翅目昆虫的研究中有诸多报道。通过携带靶标昆虫基因片段的重组病毒载体侵染寄主植物,病毒在复制过程中形成dsRNA。实验昆虫取食被侵染的寄主植物后会摄入大量的dsRNA,从而达到dsRNA导入的目的。与其它dsRNA导入方法相比,VIGS技术具有高效、高通量、昆虫接受度高等优势。本文综合分析了VIGS技术的作用机理、应用研究、影响效率的因素及其优缺点,将来应围绕这些问题进一步深化该技术在昆虫学研究中的应用。
RNA interference has been extensively applied to research on insect gene function.Successful application of this technology requires accurate delivery of interfering agents,such as double-stranded RNA(dsRNA),or short interfering RNA(siRNA),to target organisms.This paper focuses on a unique strategy for delivering dsRNA into plant-feeding insects,namely VIGS(virus-induced gene silencing)technology.Most studies that have used VIGS to investigate gene function have focused on hemipteran and lepidopteran pests.A gene fragment targeting a plant-feeding insect is inserted into the genome of a recombinant virus,which then infects the host plant.Double-stranded RNA can be produced in the host plant through virus replication.When an insect feeds on the host plant,dsRNA is ingested to inhibit the expression of the target gene.Due to the complex interactions among viruses,plants,and insects,several factors can affect the efficiency of this system.In comparison to other dsRNA introducing methods,VIGS technology is less labor-intensive and allows the large-scale screening of genes.Moreover,it has proven highly efficient in suppressing some lepidopteran insect pests that are reported to be refractory to RNAi.The application of VIGS technology to insect RNAi has,however,some limitations.This paper provides a reference for the further development and application of VIGS technology in insect research and pest control,and provides an overview of the mechanisms,current applications,and factors affecting suppression efficiency.The advantages and problems associated with this technology are also discussed so that these can be addressed in future research.
作者
王鑫仪
靖湘峰
WANG Xin-Yi;JING Xiang-Feng(State Key Laboratory of Crop Stress Biology for Arid Areas,Northwest A&F University,Yangling 712100,China)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2021年第3期508-519,共12页
Chinese Journal of Applied Entomology
基金
国家重点研发计划(2017YFD0200400)。
