摘要
目的:探讨运脾颗粒对功能性消化不良(functional dyspepsia,FD)小鼠胃肠动力和胃泌素(gastrin,GAS)、抑胃肽(gastric inhibitory polypeptide,GIP)的影响。方法:将SPF级KM种雄性小鼠100只随机抽取25只作为空白对照组,正常饲养,其余小鼠混合饲养,采取饥饱失常和夹尾激怒法造模。将造模成功后的75只小鼠恢复正常喂食,并随机分为模型对照组、运脾颗粒组和吗丁啉组。空白对照组和模型对照组用生理盐水灌胃14天,运脾颗粒组和吗丁啉组分别灌胃相应药物14天,4组小鼠在最后1次灌胃后禁食12 h,于第2日灌活性碳半固体营养糊1 mL,30 min后断头取血,取出小鼠胃和小肠,计算胃内残留率和小肠推进率,并用酶联免疫吸附法(enzyme linked immunosorbent assay,ELISA)测定小鼠血清GAS和GIP含量。结果:1)运脾颗粒能明显改善FD小鼠皮毛、饮食、精神、活动等一般情况。2)与空白对照组相比,模型对照组小鼠胃内残留率较高,小肠推进减弱,差异有统计学意义(P<0.05);与模型对照组比较,运脾颗粒组和吗丁啉组小鼠胃内残留率降低,小肠推进率增大,差异有统计学意义(P<0.05);运脾颗粒组和吗丁啉组相比,胃内残留率和小肠推进率差异无统计学意义(P>0.05)。3)与空白对照组对比,模型对照组血清胃泌素减少,抑胃肽升高,差异有统计学意义(P<0.05);与模型组比较,运脾颗粒组和吗丁啉组血清胃泌素增高,抑胃肽降低,差异有统计学意义(P<0.05);运脾颗粒组和吗丁啉组相比差异无统计学意义(P>0.05)。结论:运脾颗粒可以促进胃排空和加快小肠运动,作用机制可能与增加胃泌素和降低抑胃肽有关。
To explore the effects of spleen-activating granules on gastrointestinal motility,GAS and GIP in FD mice.Methods:All 25 mice were randomly selected as blank control group among 100 SPF KM male mice and they were fed normally,the rest of the mice were raised and prepared into the models by means of hunger and satiety disorder and tail clamping and infuriation,after successful modeling and returning to normal feeding,75 mice were randomized into model control group,spleen-activating granules group and motilium group.Blank control group and model control group were drenched for 14 days with physiological saline,spleen-activating granules group and motilium group for 14 days with the corresponding drugs respectively,the mice in four groups fasted for 12 hours after the last gavage,perfused with activated carbon semi solid nutrition paste on the second day,1mL,and sacrificed to draw blood in 30 minutes,to take out the stomach and small intestine of the mice,to calculate gastric residual rate and small intestinal propulsion ratio,ELISA was used to measure the contents of GAS and GIP.Results:1)Spleen-activating granules can significantly improve the general conditions of FD mice,such as fur,diet,spirit and activity.2)Compared with blank control group,the mice showed higher residual rate in the stomach and poor small intestinal propulsion rate in model control group,and the difference had statistical meaning(P<0.05);compared with model control group,gastric residual rates of spleen-activating granules group and motilium group lowered,and small intestinal propulsion rate increased and the difference was statistically significant(P<0.05);the difference had no statistical meaning when spleen-activating granules group was compared with motilium group in gastric residual rate and small intestinal propulsion rate(P>0.05).3)Compared with blank control group,GAS reduced while GIP rose in model control group,and the difference showed statistical meaning(P<0.05);compared with the model group,while GIP lowered in spleen-activating granules group and motilium group,and the difference had statistical meaning(P<0.05);the difference had no statistical meaning when spleen-activating granules group was compared with motilium group(P>0.05).Conclusion:Spleen activating granules could promote gastric emptying and intestinal motility,and the mechanism might be related to increasing GAS and reducing GIP.
作者
陈燕云
李初谊
李竞
CHEN Yanyun;LI Chuyi;LI Jing(China People’s Liberation Army No.96604 Hospital,Lanzhou 730000,China;Hospital 940 of PLA Joint Logistics Support Force;Gansu Provincial Hospital of Traditional Chinese Medicine)
出处
《西部中医药》
2021年第7期44-47,共4页
Western Journal of Traditional Chinese Medicine
