摘要
目的以链霉菌Streptomyces sp.CPCC 204095为产生菌,建立小规模发酵培养及分离纯化制备原黄醇酮C的方法。方法HPLC测定原黄醇酮C发酵效价;利用改进的发酵培养基配方对链霉菌Streptomyces sp.CPCC 204095进行固态发酵培养,经乙酸乙酯提取、正相硅胶色谱柱和反相ODS色谱柱分离纯化制备原黄醇酮C。结果采用改进的发酵培养基配方(葡萄糖4 g/L,麦芽提取物25 g/L,酵母提取物4 g/L,黄豆饼粉6 g/L,琼脂粉15 g/L,pH自然),原黄醇酮C发酵效价达到300 mg/L;从5 L发酵培养基出发,经发酵培养和分离纯化后,收获原黄醇酮C样品656 mg,HPLC检测纯度91%。结论建立了一种简便高效的原黄醇酮C小规模发酵和制备工艺流程,为开展原黄醇酮C化学衍生物和生物活性等研究奠定了基础。
Objective To establish an efficient procedure for small scale fermentation and preparation of isatropolone C from Streptomyces sp. CPCC 204095. Methods Solid state fermentation of Streptomyces sp. CPCC 204095 was carried out for isatropolone C production. HPLC was used to determine the titers of isatropolone C. Isolation and purification of isatropolone C was accomplished by EtOAc extraction, followed by silica gel column chromatography and ODS column chromatography. Results Streptomyces sp. CPCC 204095 produced isatropolone C at the level of 300 mg/L when it was cultured in a selective solid state fermentation medium composed of glucose 4 g/L, malt extract 25 g/L, yeast extract 4 g/L, soybean meal 6 g/L, and 15 g/L agar. Following the isolation and purification procedure, an amount of 656 mg isatropolone C with 91% purity was obtained from 5 L fermentation culture of Streptomyces sp. CPCC 204095. Conclusion A simple and efficient procedure was established for isatropolone C production at a small scale, which will provide enough amounts of isatropolone C for its future semi-synthesis and biological activity studies.
作者
刘晓岩
李书芬
黎林丽
刘佳昌
江冰娅
武临专
LIU Xiao-yan;LI Shu-fen;LI Lin-li;LIU Jia-chang;JIANG Bing-ya;WU Lin-zhuan(NHC Key Laboratory of Biotechnology of Antibiotics,CAMS Key Laboratory of Synthetic Biology for Drug Innovation,Institute of Medicinal Biotechnology,Chinese Academy of Medical Sciences&Peking Union Medical College,Beijing 100050,China)
出处
《中国医药生物技术》
2021年第4期302-306,共5页
Chinese Medicinal Biotechnology
基金
国家重点研发计划(2018YFA0902000)
国家微生物资源基础平台(NIMR-2018-3)
中国医学科学院医学与健康科技创新工程(2016-I2M-3-012)。
