摘要
为了解小麦穗发芽的遗传机制,发掘与小麦穗发芽相关的候选基因,采用整穗发芽法对来自全国的207份小麦品种(系)进行表型鉴定,并结合小麦90K SNP基因芯片,通过TASSLE软件的MLM (Q+K)模型对小麦的整穗发芽率进行全基因组关联分析(genome-wide association study, GWAS)。研究结果表明,不同年份间小麦品种(系)的穗发芽表现出丰富的表型变异,变异系数为0.34和0.25,多态性信息含量(polymorphicinformationcontent,PIC)为0.01~0.38,全基因组LD衰减距离为3 Mb。群体结构分析和主成分分析表明, 207份小麦品种(系)所构成的自然群体结构简单,可分为3个亚群。GWAS检测结果显示,在不同环境间共检测到34个与小麦穗发芽显著关联的SNP标记位点(P≤0.001),分布在小麦3A、3B、4A、4B、5D、6A、6B、6D、7B和7D染色体上,单个位点可解释5.55%~11.63%的表型变异, 16个标记位点在两个及以上环境下均被检测到,其中6B染色体上的标记wsnp_Ex_c14101_22012676在E1、E2及平均环境下被共同检测到,属稳定遗传的位点。通过对表型效应值大且稳定遗传的关联位点进行挖掘,共筛选到13个与小麦穗发芽相关的候选基因。其中TraesCS3A01G589400LC、TraesCS6B01G138600/TraeCS6B01G516700LC/TraesCS6B01G548900LC、TraesCS6D01G103600及TraesCS7B01G200100等基因通过调控植物内源激素-脱落酸(abscisicacid,ABA)的灵敏性进而影响种子的休眠;TraesCS3B01G415900LC、 TraesCS6A01G144700LC及TraesCS6B01G294800等基因编码的F-box蛋白在植物激素的信号转导、光信号转导以及花器官发育等生理过程中起重要作用;TraesCS6A01G108800、TraesCS6B01G138200/TraesCS6B01G293700基因编码Myb转录因子家族蛋白能调控种子中类黄酮的生物合成,对籽粒颜色有重要影响,这些候选基因是与小麦穗发芽相关的重要基因。
To understand the genetic mechanism of wheat pre-harvest sprouting(PHS)in wheat breeding,it is significant to explore marker loci and candidate genes associated with PHS resistance using intact spikes.In this study,a total of 207 wheat varieties(lines)from China and 16,686 SNP markers were analyzed in wheat whole genome.The mixed liner model(Q+K)was used to analyze PHS phenotypic data in three environments.Genome-wide association study showed that there were abundant phenotypic variations in different environments and wheat varieties(lines).The coefficient of variation was 0.34 and 0.25,the polymorphic information content of value(PIC)was from 0.01 to 0.38,and the attenuation distance of whole genome LD was 3 Mb.The population structure and principal component analysis revealed that 207 wheat varieties(lines)could be divided into three subgroups.GWAS results indicated that 34 SNP markers were detected,which were significantly associated with pre-harvest sprouting at P<0.001.They were located on chromosomes 3 A,3 B,4 A,4 B,5 D,6 A,6 B,6 D,7 B,and 7 D,and each explained 5.55%–11.63%of phenotypic variation.There were 16 markers loci detected in more than two environments,and the marker NpExc1410122,012,676 on 6 B chromosome detected in E1,E2,and average environment.Meanwhile,13 candidate genes were screened out by mining association loci with large phenotypic effect value and stable inheritance.Traes CS3A01G589400LC,TraesCS6B01G138600/TraesCS6B01G516700LC/TraesCS6B01G548900LC,Traes CS6D01G103600,and TraesCS7B01G200100 could affect seed dormancy by regulating the sensitivity of endogenous ABA in plants.The F-box proteins were encoded by Traes CS3B01G415900LC,Traes CS6A01G144700LC,and TraesCS6B01G294800,which played major roles in plant hormone signal transduction,light signal transduction,and flower organ development.TraesCS6A01G108800,TraesCS6B01G138200/TraesCS6B01G293700 encoded Myb transcription factor family.These candidate genes are important genes related to wheat sprouting.
作者
谢磊
任毅
张新忠
王继庆
张志辉
石书兵
耿洪伟
XIE Lei;REN Yi;ZHANG Xin-Zhong;WANG Ji-Qing;ZHANG Zhi-Hui;SHI Shu-Bing;GENG Hong-Wei(Agricultural College of Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China;Key Laboratory of Biotechnology,Xinjiang Agricul-tural University,Urumqi 830052,Xinjiang,China;Institute of Grain Crops,Xinjiang Academy of Agricultural Sciences,Urumqi 830091,Xinjiang,China)
出处
《作物学报》
CAS
CSCD
北大核心
2021年第10期1891-1902,共12页
Acta Agronomica Sinica
基金
国家自然科学基金项目(31771786)
新疆维吾尔自治区科技创新基地建设项目(PT1910)资助。
关键词
小麦
穗发芽
全基因组关联分析
SNP
候选基因
wheat
pre-harvest sprouting
genome-wide association analysis
SNP
candidate gene
