摘要
目的研究槲皮素对Tca-8113细胞增殖、迁移和侵袭功能的影响及PI3K/AKT通路的调控作用。方法体外培养Tca-8113细胞,将细胞分为对照组和槲皮素(50μmol/L)处理组。采用CCK-8实验检测细胞增殖能力;Transwell(添加基质胶)检测细胞迁移(侵袭)情况;实时荧光定量PCR(qRT-PCR)检测细胞中PTEN、AKT、FOXO1以及BCL2L11 mRNA表达,蛋白质免疫印迹法(Western blot)检测FOXO1、p-FOXO1、AKT、p-AKT、PTEN和BCL2L11蛋白表达。结果与对照组相比,槲皮素处理组Tca-8113细胞的增殖、迁移及侵袭能力显著降低,PTEN和BCL2L11 mRNA和蛋白表达水平均显著上调(P<0.05),FOXO1和AKT磷酸化水平显著下调(P<0.01),但AKT和FOXO1 mRNA表达水平无显著差异。结论槲皮素可抑制Tca-8113细胞增殖、迁移及侵袭,其机制可能是槲皮素促进PTEN的表达,负调控PI3K/AKT通路减弱FOXO1的磷酸化水平,引起BCL2L11的表达增加实现的。
Objective To investigate the effect of quercetin on cell proliferation,migration,and invasion and PI3K/AKT pathway regulation in the oral cancer cell line Tca-8113.Methods Tca-8113 cells were cultured in vitro and divided into a control group and quercetin(50μmol/L)treatment group.A CCK-8 assay was used to detect cell proliferation;a Transwell assay(with Matrigel)was used to detect cell migration(invasion);quantitative real-time polymerase chain reaction was used to detect the expression of PTEN,AKT,FOXO1,and BCL2L11 mRNA;and Western blot was used to detect FOXO1,p-FOXO1,AKT,p-AKT,PTEN,and BCL2L11 protein expression.Results Compared with the control group,the proliferation,migration,and invasion abilities of Tca-8113 cells in the quercetin treatment group were significantly reduced;the PTEN and BCL2L11 mRNA and protein expression levels were significantly up-regulated(P<0.05);and the phosphorylated FOXO1 and AKT levels were significantly down-regulated(P<0.01).However,there was no significant difference in the AKT and FOXO1 mRNA expression levels between the two groups.Conclusions Quercetin can inhibit the proliferation,migration,and invasion of Tca-8113 cells.In terms of the underlying mechanism,quercetin may promote the expression of PTEN and negatively regulate the PI3K/AKT pathway to reduce the phosphorylation level of FOXO1,causing an increase in the expression of BCL2L11.
作者
肖兰飞
高继萍
闫晓如
王晓堂
续国强
宋国华
XIAO Lanfei;GAO Jiping;YAN Xiaoru;WANG Xiaotang;XU Guoqiang;SONG Guohua(Laboratory Animal Center,Shanxi Medical University;Shanxi Key Laboratory of Experimental Animal Science and Human Disease Animal Models,Taiyuan 030001,China.2.Mental Health School Affiliated to Shanxi Medical University,Taiyuan 030001)
出处
《中国比较医学杂志》
CAS
北大核心
2021年第6期30-34,共5页
Chinese Journal of Comparative Medicine
基金
国家自然科学基金(31772551)。
