摘要
【目的】松毛虫赤眼蜂Trichogramma dendrolimi是一种用于鳞翅目害虫生物防治的重要卵期寄生蜂。本研究旨在明确孤雌产雌生殖品系松毛虫赤眼蜂的产卵强度对其体内Wolbachia滴度及Wolbachia诱导的孤雌产雌生殖表型的影响。【方法】在室内调查了3个处理组松毛虫赤眼蜂雌蜂不同产卵强度(每日仅1 h供寄主卵、隔日24 h供寄主卵和持续供寄主卵)对其生物学指标包括7 d内逐日子代雄性比、逐日产卵量、累积子代雄性比和累积产卵量的影响;采用实时荧光定量PCR方法检测未产卵雌蜂(对照)以及每日仅1 h供寄主卵和持续供寄主卵的雌蜂体内Wolbachia滴度(wsp基因拷贝数)。【结果】持续供寄主卵的雌蜂累积子代雄性比显著高于每日仅1 h供寄主卵,但与隔日24 h供寄主卵相比无显著差异。3个处理组雌蜂逐日子代雄性比随日龄的增加均显著上升,其中持续供寄主卵的处理组的上升幅度最高。持续供寄主卵的雌蜂累计产卵量显著高于每日仅1 h供寄主卵和隔日24 h供寄主卵。3个处理组雌蜂逐日产卵量随日龄的增加而显著下降,其中持续供寄主卵的处理组的下降幅度最高。未产卵的雌蜂体内Wolbachia滴度显著高于持续提供寄主卵的雌蜂体内的滴度,但与每日仅1 h供寄主卵的雌蜂体内的滴度相比无显著差异。【结论】结果说明,当松毛虫赤眼蜂雌蜂产卵不受限制时,Wolbachia滴度减少,宿主孤雌产雌表型减弱;而限制雌蜂产卵有助于维持Wolbachia滴度和宿主孤雌产雌表型。研究结果为认识Wolbachia与宿主赤眼蜂之间的互作以及应用孤雌产雌生殖品系松毛虫赤眼蜂防控害虫提供一定的参考。
【Aim】Trichogramma dendrolimi is an important egg parasitoid applied in the biological control of lepidopteran pests.This study aims to investigate the effects of oviposition intensity on the titer of Wolbachia in parthenogenetic T.dendrolimi and its parthenogenetic reproductive phenotype induced by Wolbachia.【Methods】The effects of different oviposition intensities of females of T.dendrolimi in three treatment groups(supplied with host eggs for only 1 h per day,supplied with host eggs for 24 h every other day and supplied with host eggs all the time)on its biological parameters including the daily proportion of offspring males,daily fecundity,cumulative proportion of offspring males,and cumulative fecundity within 7 d were investigated in the laboratory.Besides,the Wolbachia titers(the copy numbers of wsp)in T.dendrolimi females without oviposition experience(the control),supplied with host eggs for only 1 h per day and supplied with host eggs all the time were detected by fluorescence quantitative real-time PCR.【Results】The cumulative proportion of offspring males of T.dendrolimi females supplied with host eggs all the time was significantly higher than that of females supplied with host eggs for only 1 h per day,but was not significantly different from that of females supplied with host eggs for 24 h every other day.The daily proportions of offspring males in the three treatment groups significantly increased with wasp age,and the increase rate was the highest in the treatment group of females supplied with host eggs all the time.The cumulative fecundity of T.dendrolimi females supplied with host eggs all the time was significantly higher than those of females supplied with host eggs for only 1 h per day and females supplied with host eggs for 24 h every other day.The daily fecundity in the three treatment groups significantly decreased with wasp age,but the decrease rate was the highest in the treatment group of females supplied with host eggs all the time.The Wolbachia titer in T.dendrolimi females without oviposition experience was significantly higher than that in T.dendrolimi females supplied with host eggs all the time,but showed no significant difference from that in T.dendrolimi females supplied with host eggs for only 1 h per day.【Conclusion】The results suggest that both Wolbachia titer and parthenogenetic phenotype of T.dendrolimi decline when its females can deposit their eggs without limitation.Limiting time for oviposition will be helpful to maintain Wolbachia titer and parthenogenetic phenotype.The results provide references for understanding the interaction between Wolbachia and host Trichogramma and the application of thelytokous parthenogenetic T.dendrolimi for controlling pest insects.
作者
霍梁霄
李媛媛
张丹
于茜
宁素芳
赵旭
周金成
董辉
HUO Liang-Xiao;LI Yuan-Yuan;ZHANG Dan;YU Qian;NING Su-Fang;ZHAO Xu;ZHOU Jin-Cheng;DONG Hui(College of Plant Protection, Shenyang Agricultural University, Shenyang 110161, China;Liaoning Agricultural Development Service Center, Shenyang 110034, China)
出处
《昆虫学报》
CAS
CSCD
北大核心
2021年第5期597-604,共8页
Acta Entomologica Sinica
基金
国家重点研发计划(2017YFD0201805)
辽宁省自然科学基金项目(2020-BS-137)。
关键词
沃尔巴克氏体
松毛虫赤眼蜂
孤雌产雌生殖
产卵强度
产卵量
Wolbachia
Trichogramma dendrolimi
thelytokous parthenogenesis
oviposition intensity
fecundity
