摘要
为发掘具有防治茶园杂草潜力的生防菌,本研究从江苏、浙江和安徽3省共16个茶园采集感病杂草,采用组织分离法分离出病原菌,通过菌饼回接验证获得了44株具有致病力的植物病原菌,从中筛选出了10株致病性较强的优势菌株。利用柯赫氏法则验证优势菌株后,通过观察优势病原菌的形态特征,结合rDNA-ITS基因片段序列分析,确定10株优势菌株分别为平脐蠕孢属的狗尾草平脐蠕孢Bipolaris setariae、链格孢属的链格孢霉Alternariaalternata、弯孢属的间型弯孢Curvulariaintermedia和新月弯孢Curvularialunata、镰刀属的再育镰刀菌Fusariumproliferatum和木贼镰刀菌Fusariumequiseti、棒孢属的多主棒孢Corynesporacassiicola和茎点霉属的荸荠茎点霉Didymellabellidis,共6属8种的病原真菌。结合作物安全性问题,木贼镰刀菌、再育镰刀菌、狗尾草平脐蠕孢、间型弯孢和新月弯孢5种病原菌被认为具有开发成防除茶园杂草生物除草剂的较好潜力。
To screen out plant pathogens with strong pathogenicity to weeds and high safety to tea plant,the diseased weeds were collected from 16 tea gardens in Jiangsu,Zhejiang and Anhui provinces.Forty-four plant pathogens were isolated,thereinto,10 fungus strains with high pathogenicity were selected and further tested by Koch’s postulates.Based on morphological characteristics and sequence analysis of rDNA-ITS genes,these ten stains were identified as Bipolaris setariae,Alternaria alternata,Curvularia intermedia and C.lunata,Fusarium proliferatum and F.equiseti,Corynespora cassiicola and Didymella bellidis,belonging to 8 different species of pathogenic fungi.Considering crop safety,five candidate strains,F.equiseti,F.proliferatum,B.setariae,C.intermedia and C.lunata,exhibit the good potential for development into bioherbicides in weed control in tea garden.
作者
陆俞萍
肖婉
房婉萍
强胜
陈世国
LU Yuping;XIAO Wan;FANG Wanping;QIANG Sheng;CHEN Shiguo(Weed Research Laboratory,Nanjing Agricultural University,Nanjing 210095,China;Tea Science Research Institute,Nanjing Agricultural University,Nanjing 210095,China)
出处
《中国生物防治学报》
CSCD
北大核心
2021年第3期564-574,共11页
Chinese Journal of Biological Control
基金
国家重点研发计划(2017YFD0201300)
国家现代茶叶产业技术体系(CARS-19)
江苏省重点研发计划(BE2019379)。
关键词
茶
病原菌
分离与鉴定
生物除草剂
tea plant
pathogen
isolation and identification
bioherbicide
