摘要
作为板栗加工的副产物,板栗皮含有以儿茶素类物质为代表的多酚化合物,具有抗氧化、抑菌以及抗肿瘤等作用。为研究板栗皮中活性物质的生物利用率,本文采用超高效液相色谱-串联质谱(UPLC-MS/MS)的广泛靶向代谢组学技术,研究了板栗皮儿茶素类物质经过模拟消化体系后的组成变化。无监督模式识别的主成分分析(PCA)结果表明,消化前后板栗皮样品中的儿茶素类物质含量存在显著差异。方差分析及聚类热图结果显示,在经过模拟消化体系后,板栗皮中的8种儿茶素类物质含量均显著降低,其中降低程度较大的是没食子儿茶素、表没食子酸儿茶素以及表儿茶素,在板栗外皮中分别降低了94.96%、89.70%及87.77%,板栗内皮中分别降低了95.20%、90.71%及90.61%。综上,板栗皮儿茶素类物质在消化过程中稳定性较差,未来的研究中可通过负载等方式对其进行包埋,以提高其生物利用率。
As a by-product of chestnut processing,chestnut peel contains polyphenols represented by catechins,which has antioxidant,bacteriostatic and anti-tumor effects.In order to study the bioavailability of active substances in chestnut peel,widely targets metabonomics technique based on UPLC-MS/MS was used to study the composition changes of catechins in chestnut peel before after simulated digestion.The results of PCA with unsupervised pattern recognition showed that there were significant differences in the contents of catechins between chestnut peel samples before and after digestion.The results of variance analysis and cluster heat map showed that the contents of eight catechins in chestnut peel decreased significantly after the simulated digestion system,among which,the contents of epigallocatechin,epigallocatechin and epigallocatechin decreased to a higher degree.In conclusion,chestnut bark catechins have poor stability during digestion,and can be embedded by loading in future studies to improve the fraction of bioavailability.
作者
雷嗣超
张家音
赵泓涛
李浩楠
杨芳
LEI Si-chao;ZHANG Jia-yin;ZHAO Hong-tao;LI Hao-nan;YANG Fang(School of Environmental Ecology and Biological Engineering,Wuhan Institute of Technology,Wuhan 430205,China)
出处
《中国果菜》
2021年第6期105-111,共7页
China Fruit & Vegetable
基金
湖北省大学生创新创业训练计划资助项目(S202010490074)
武汉工程大学校长基金资助项目(2020155、2020151)。
关键词
板栗皮
儿茶素
体外消化
差异代谢物
主成分分析
聚类分析
Chestnut skin
catechin
in vitro digestion
differential metabolites
principal component analysis
cluster analysis
