摘要
【目的】探讨新加香薷饮合柴葛解肌汤加减治疗登革热的分子机理。【方法】收集广州市花都区人民医院2018年1月至12月登革热患者6例(治疗前设为登革热组,治疗后设为中药治疗组),另收集正常人6例作为正常对照组。应用转录组测序(RNA-seq)技术检测各组mRNA表达情况,比较登革热组与正常对照组获得差异基因,比较中药治疗组与登革热组获得差异基因,再获得两者的交集基因,进行表达模式分析、功能富集分析以及蛋白与蛋白相互作用网络分析以获取起关键作用的基因。【结果】筛选到登革热组与正常对照组比较的差异基因2 704个,中药治疗组与登革热组比较的差异基因882个,筛选出中药逆转的潜在靶基因279个。基因本体论(GO)、京都基因与基因组百科全书(KEGG)信号通路富集分析发现,这些基因大多参与了炎症、自身免疫应答相关功能,如干扰素应答反应、维甲酸诱导基因I(RIG-I)样受体信号通路和T细胞活化的调节。另外,通过蛋白与蛋白网络分析确定了这些基因间的相互作用关系并筛选到45个可能起关键作用的基因,如IRF7、CCL2、IL15等。【结论】新加香薷饮合柴葛解肌汤加减治疗登革热的机制可能与其调节炎症、自身免疫应答相关的基因和信号通路有关。
Objective To explore the molecular mechanism of modified Xinjia Xiangru Yin plus Chai Ge Jieji Tang for dengue fever. Methods Six cases of patients with dengue fever were admited in Guangzhou Huadu District People’s Hospital form January to December,2018,served as dengue fever group before treatment and Chinese medicine treatment group after treatment,additionally,6 cases of healthy humans served as normal control group.RNA sequencing was used to detect the mRNA expression in various groups,and then the differentially expressed genes were gained by comparison of the dengue fever group and normal group, and of the Chinese medicine treatment group and normal control group, respectively. Furthermore, the intersection of the achieved genes between the two groups was obtained to perform expression pattern analysis, function enrichment analysis and protein-protein interaction network analysis for obtaining the key genes. Results A total of 2 704 differentially expressed genes were screened out between the dengue fever group and normal group, and 882 differentially expressed genes were screened out between the Chinese medicine treatment group and dengue fever group,and finally 279 potential target genes reversed by Chinese medicine were obtained. The enrichment analysis results of Gene Ontology(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)signaling pathway showed that most of these genes were involved in inflammation and autoimmune response related functions, such as interferon response, retinoic acid-inducible gene I(RIG-I)-like receptor signaling pathway and regulation of T cell activation. In addition,the interaction among these genes was determined by protein-protein interaction network analysis, and 45 genes, such as CCL2, CXCL1, IL15, which might play a key role, were screened out.Conclusion The mechanism of modified Xinjia Xiangru Yin plus Chai Ge Jieji Tang for dengue fever may be related to regulating the genes and signal pathways related to inflammation and autoimmune response.
作者
钟小兰
廖小明
沈菲
于海建
谭丽娟
ZHONG Xiao-Lan;LIAO Xiao-Ming;SHEN Fei;YU Hai-Jian;TAN Li-Juan(Guangzhou Huadu District People's Hospital,Guangzhou 510800 Guangdong,China)
出处
《广州中医药大学学报》
CAS
2021年第6期1208-1213,共6页
Journal of Guangzhou University of Traditional Chinese Medicine
基金
广东省中医药局中医药科研项目(编号:20191257)。
关键词
新加香薷饮
柴葛解肌汤加减
登革热
转录组测序
炎症
自身免疫应答
Xinjia Xiangru Yin
Chai Ge Jieji Tang
dengue fever
RNA sequencing(RNA-seq)
inflammation
antoimmune response
