摘要
为了解大伙房水库生态功能特性和有效改善其水环境质量,2015年5月至2016年4月,在大伙房水库布点采集水样,应用高通量测序技术结合典范对应分析(canonical correspondence analysis,CCA)方法,在为期1年的时间内,研究了该水库4个季节细菌的群落结构及其多样性差异与水体环境因子之间的变化关系。结果表明,全年大伙房水库细菌群落Shannon-Wiener指数平均达7.312,存在季节分布差异,秋季指数最高,冬季指数最低,序列比对中,发现64个样本中的细菌种类覆盖54门、135纲、220目、279科、412属。变形菌门(Proteobacteria)、蓝菌门(Cyanobacteria)、拟杆菌门(Bacteroidetes)和放线菌门(Actinobacteria)为优势菌门,优势菌属为Fluviicola、聚球藻属(Synechococcus)、黄杆菌属(Flavobacterium)和短波单胞菌属(Brevundimonas),不同季节的大伙房水库水体中微生物群落结构变化较大。CCA分析结果表明,溶氧(DO)、pH、温度(T)、总磷(TP)是影响细菌群落结构及其分布的主要环境驱动因子。
In order to understand the ecological function characteristics of Dahuofang Reservoir and effectively improve its water environment quality,the authors collected water samples from deployed sites of the reservoir from May 2015 to April 2016,and applied high throughput sequencing technology combined with canonical correspondence analysis method(CCA)to study the differences of bacterial community structure,biodiversity and their variational relationship with water environmental factors in Dahuofang Reservoir within four seasons of a year period.The results showed that the average Shannon-Wiener index of bacterial community in Dahuofang reservoir was 7.312,existed seasonally distributed differences,the index in autumn was at the highest,and the index in winter was at the lowest.It was found in sequences matching that the bacterial species in the 64 samples covered 54 phyla,135 classes,220 orders,279 families,and 412 genera.The dominant bacteria phyla were Cyanobacteria,Proteobacteria,Bacteroidetes,Actinobacteria,and the dominant genera were Fluviicola,Synechococcus,Flavobacterium,and Brevundimonas,and the structure of microbial community changed comparatively great in different season in waters of Dahuofang reservoir.The results of CCA analyses showed that dissolved oxygen(DO),pH,temperature,and total phosphorus were the main environmental driving factors that affected the structure and distribution of bacteria community.
作者
邵缨迪
赵文
尹东鹏
王美儒
邹京京
杨凤山
徐锋
王欢
SHAO Ying-di;ZHAO Wen;YIN Dong-peng;WANG Mei-ru;ZOU Jing-jing;YANG Feng-shan;XU Feng;WANG Huan(Key Lab.of Hydrobiol.in Liaoning Prov.,Coll,of Fish.&Life Sci.,Dalian Ocean Uni.,Dalian 116023;Liaoning Runzhong Water Supply Co.,Ltd.,Shenyang 110166;Liaoning Dahuofang Fish.Co.,Ltd.,Fushun 113003)
出处
《微生物学杂志》
CAS
CSCD
2021年第1期33-42,共10页
Journal of Microbiology
基金
横向联合项目(2015-2018)。
关键词
细菌群落
多样性
16S
rDNA环境驱动因子
大伙房水库
bacterial community
biodiversity
16S rDNA
environmental driving factors
Dahuofang reservoir
