摘要
以冬瓜子叶节为外植体,研究不同激素配比和暗培养天数对子叶节愈伤诱导再生芽的影响,不同浓度激素组合对冬瓜芽继代扩繁的影响,以及再生苗的生根与驯化移栽,建立了完整的冬瓜子叶节再生体系。根据正交试验结果,诱导不定芽分化的最优配比组合为MS+2,4-D 0.1 mg·L^(-1)+IBA 0.10 mg·L^(-1)+6-BA 0.8 mg·L^(-1)暗培养7 d,暗培养天数和2,4-D浓度对诱导再生影响较大;冬瓜芽继代扩繁最佳培养基为MS+NAA 0.05 mg·L^(-1)+6-BA 0.2 mg·L^(-1),平均增殖系数为2.487;在生根培养基1/2MS+NAA 0.2 mg·L^(-1)上再生苗生根率为100%,其驯化移栽的成活率达到80%以上。
In this study,taking cotyledon nodes as explants,the effects of different proportions and concentrations of hormones and days of dark culture on cotyledon node callus-induced regeneration buds were optimized,the effect of different concentrations of hormone combinations on adventitious bud proliferation were studied,and the rooting and transplanting of regenerated seedlings were tested,finally we established a relatively comprehensive set of regeneration system of wax gourd cotyledon nodes.According to the result of orthogonal experiment,the optimum combination for inducing adventitious bud differentiation was MS+2,4-D 0.1 mg·L^(-1)+IBA 0.10 mg·L^(-1)+6-BA 0.8 mg·L^(-1) after 7 days dark culture;and the time of dark culture and concentration of 2,4-D were major factors affecting regeneration.The best medium for proliferation of adventitious bud was MS+NAA 0.05 mg·L^(-1)+6-BA 0.2 mg·L^(-1),with a proliferation coefficient of 2.487.The rooting rate of the regenerated seedlings on the rooting medium was 100%on 1/2MS+NAA 0.2 mg·L^(-1) and the survival rate of the domesticated seedlings was more than 80%after transplanting.
作者
李志芳
闫晋强
韩向峰
谢大森
刘文睿
伍倩慧
江彪
LI Zhifang;YAN Jinqiang;HAN Xiangfeng;XIE Dasen;LIU Wenrui;WU Qianhui;JIANGBiao(Foshan Institute of Agricultural Sciences,Foshan 528145,Guangdong,China;Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,Guangdong,China)
出处
《中国蔬菜》
北大核心
2021年第4期59-63,共5页
China Vegetables
基金
广东省重点领域研发计划项目(2020B020220003)
广东省自然科学基金项目(2018A030313495)
国家自然科学基金项目(31972403,31801851)
广州市珠江科技新星专项(201806010030)
中国博士后基金项目(2020M672555)。
关键词
冬瓜
子叶节
组织培养
再生体系
wax gourd
cotyledon nodes
tissue culture
regeneration system
