摘要
目的探讨外周血自噬相关炎症因子表达水平与代谢综合征(MS)的相关性。方法选取2018年7月—2019年6月于中国人民解放军陆军第958医院收治的MS患者96例作为研究组,另选取同期在该院进行健康体检的人群96例作为对照组,对比两组体格检查指标、胰岛素抵抗(IR)、空腹血糖、甘油三酯、总胆固醇、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇和白介素-1β水平,并测定两组自噬相关炎症因子LC3、p62和Atg5蛋白表达水平,并分析其与MS患者临床指标的相关性。结果研究组Atg5、LC3-Ⅱ/LC3-Ⅰ的表达水平明显高于对照组(P<0.05),p62表达水平明显低于对照组(P<0.05);相关性分析结果显示,LC3-Ⅱ/LC3-Ⅰ和Atg5的表达与体重指数、收缩压、舒张压、空腹血糖、总胆固醇、甘油三酯、低密度脂蛋白胆固醇、IR和白介素-1β均呈正相关性(P<0.05),与高密度脂蛋白胆固醇呈负相关性(P<0.05);而自噬相关炎症因子p62则相反;进一步多元线性回归分析发现,LC3表达量的重要相关因素为IR、甘油三酯和白介素-1β;p62和Atg5表达量的重要相关因素为IR和白介素-1β。结论MS患者自噬相关炎症因子LC3、p62和Atg5蛋白均呈现高表达,并与患者一般临床指标存在明显相关性,对于研究MS的发病机制和评估患者病情具有一定的指导价值。
Objective To explore the correlation between expression levels of autophagy-related inflammatory factors in peripheral blood and metabolic syndrome(MS).Methods A total of 96 MS patients who were admitted to The 958th Hospital of PLA Army from July 2018 to June 2019 were enrolled as study group.Another 96 healthy people who have physical examination in this hospital during the same period were enrolled as the control group.The physical examination indexes,insulin resistance(IR),levels of fasting blood glucose,triglyceride,total cholesterol,high density lipoprotein cholesterol,low density lipoprotein cholesterol and interleukin 1βwere compared between the two groups.The expression levels of autophagy-related factors(LC3,p62 and Atg5 protein)in both groups were measured,and their correlation with clinical indexes in MS patients was analyzed.Results The expression levels of Atg5 and LC3-Ⅱ/LC3-Ⅰin study group were significantly higher than those in control group(P<0.05),while expression level of p62 was significantly lower than that in control group(P<0.05).The correlation analysis results showed that expression of LC3-Ⅱ/LC3-Ⅰand Atg5 was positively correlated with body mass index,systolic blood pressure,diastolic blood pressure,fasting blood glucose,triglyceride,total cholesterol,low density lipoprotein cholesterol,IR and interleukin 1β(P<0.05),while negatively correlated with high density lipoprotein cholestero(P<0.05).The correlation between autophagy-related factor p62 and the above all indexes was on the contrary.It was found by the further multiple linear regression analysis that the important related factors of LC3 expression were IR,triglyceride and interleukin 1β.The important related factors of p62 and Atg5 expression were IR and interleukin 1β.Conclusion The autophagy-related inflammatory factors(LC3,p62 and Atg5 protein)in MS patients are all highly expressed,and are significantly correlated with the general clinical indexes,which had certain guiding value for studying the pathogenesis of MS and evaluating the patient's condition.
作者
代静澜
李朵
牟海刚
华蕾
DAI Jinglan;LI Duo;MOU Haigang;HUA Lei(Department of Cardiovascular Medicine,The 958th Hospital of PLA Army,Chongqing 400020,China)
出处
《心血管病学进展》
CAS
2021年第3期271-276,共6页
Advances in Cardiovascular Diseases
基金
重庆市卫生适宜技术推广项目(2018jstg036)。
关键词
细胞自噬
炎症因子
代谢综合征
相关性
微管相关蛋白1轻链3
Autophagy
Inflammatory factor
Metabolic syndrome
Correlation
Microtubule-associated protein 1 light chain 3
