摘要
目的:探讨长链非编码RNA(lncRNA)MNX1-AS1在非小细胞肺癌(NSCLC)组织中的表达及其临床意义。方法:收集2015年6月至2017年6月经病理组织学确诊并经手术切除的180例NSCLC患者的原发病灶及癌旁组织,采用实时荧光定量PCR(RT-qPCR)检测MNX1-AS1的表达,分析不同MNX1-AS1表达水平与NSCLC患者临床病理特征的关系,Kaplan-Meier法和Cox回归分析不同MNX1-AS1表达水平的预后情况。结果:NSCLC组织中MNX1-AS1的相对表达量显著高于癌旁组织,差异具有统计学意义(P<0.05)。MNX1-AS1的表达与NSCLC患者的肿瘤分化程度、TNM分期及淋巴结转移显著相关。MNX1-AS1高表达组患者的无进展生存期、总生存期及5年总生存率均低于低表达组(P<0.05)。Cox多因素回归分析显示,MNX1-AS1水平是影响NSCLC患者预后的独立因素。结论:MNX1-AS1在NSCLC组织中高表达并与其病理特征及预后密切相关,可能是一种NSCLC患者早期诊断及预后评估的新的生物学标志物。
Objective:To investigate the expression and clinical significance of long non-coding RNA(lncRNA)MNX1-AS1 in non-small cell lung cancer(NSCLC).Methods:The primary lesions and adjacent tissues of 180 patients with NSCLC from Jun.2015 to Jun.2017,diagnosed by histopathology and surgically removed,were collected.Real time quantitative PCR(RT-qPCR)was used to detect the expression of MNX1-AS1.The relationship between the expression of MNX1-AS1 and the clinicopathological characteristics of NSCLC patients was analyzed.Kaplan-Meier method and Cox regression analysis were used to analyze the prognosis of patients with different expression levels of MNX1-AS1.Results:The relative expression of MNX1-AS1 in NSCLC tissues was significantly higher than that in adjacent tissues(P<0.001).The expression of MNX1-AS1 was significantly correlated with tumor differentiation,TNM stage and lymph node metastasis in NSCLC patients.The progression free survival,overall survival and 5-year overall survival rate of patients with high expression of MNX1-AS1 were lower than those of patients with low expression of MNX1-AS1(P<0.05).Multivariate Cox regression analysis showed that MNX1-AS1 level was an independent prognostic factor in NSCLC patients.Conclusion:MNX1-AS1 was highly expressed in NSCLC and was closely related to its pathological characteristics and prognosis.It might be a new biomarker for early diagnosis and prognosis evaluation of NSCLC patients.
作者
刘高峰
张勇
张小贞
汤光耀
李保田
王保健
LIU Gaofeng;ZHANG Yong;ZHANG Xiaozhen(The 988th Hospital of PLA Joint Logistics Support,Henan Zhengzhou 450042,China)
出处
《河北医学》
CAS
2021年第3期486-490,共5页
Hebei Medicine
基金
河南省联合共建项目,(编号:LHGJ20190876)。
