摘要
目的分析外周血Th1、Th17细胞表达与布氏杆菌性脊柱炎术后复发的相关性。方法选取2017年1月—2020年3月河北北方学院附属第一医院骨外科收治布氏杆菌性脊柱炎患者68例作为研究对象(脊柱炎组),选取同期医院健康体检者70例为健康对照组。检测2组外周血Th1、Th17细胞比例,血清白介素-2(IL-2)、干扰素-γ(IFN-γ)、IL-17、IL-23、IL-6水平;分析影响布氏杆菌性脊柱炎术后复发的危险因素。结果与健康对照组比较,脊柱炎组患者外周血单核细胞Th17细胞比例及术前血清IL-17、IL-6、IL-23水平均升高(t/P=5.711/0.000、45.567/0.000、58.709/0.000、17.221/0.000),外周血单核细胞Th1细胞比例及血清IL-2、IFN-γ水平降低(t/P=34.024/0.000、8.991/0.000、5.948/0.000);与未复发亚组比较,复发亚组患者外周血单核细胞Th17细胞比例及术后血清IL-17、IL-6、IL-23水平升高(t/P=5.509/0.000、37.286/0.000、46.074/0.000、18.982/0.000),外周血单核细胞Th1细胞比例及血清IL-2、IFN-γ水平降低(t/P=15.960/0.000、3.450/0.001、2.712/0.000)。术后Th1、Th17细胞百分比及IL-17、IL-6、IL-23、IL-2、IFN-γ均是布氏杆菌性脊柱炎术后复发的危险因素[OR(95%CI)=2.375(1.657~3.405)、2.454(1.737~3.472)、2.273(1.661~3.109)、2.219(1.619~3.043)、2.278(1.684~3.082)、2.261(1.546~3.308)、2.337(1.624~3.364)]。结论布氏杆菌性脊柱炎术后复发患者外周血Th17细胞比例升高、Th1细胞比例降低,Th1、Th17细胞表达与布氏杆菌性脊柱炎术后恢复有密切关系。
Objective To analyze the correlation between the ratio of Th1 and Th17 cells in peripheral blood and the recurrence of brucellosis spondylitis.Methods From January 2017 to March 2020,68 patients with brucellosis spondylitis admitted to the Department of Orthopedic Surgery of the First Affiliated Hospital of Hebei North University were selected as the research object(spondylitis group),and 70 patients with healthy physical examination in the hospital during the same period were selected as the healthy control group.The proportion of Th1 and Th17 cells in the peripheral blood of the two groups was detected,and the levels of serum interleukin 2(IL-2),interferon gamma(IFN-γ),IL-17,IL-23,and IL-6 were detected.To analyze the risk factors affecting the recurrence of brucellosis spondylitis after surgery.Results Compared with the healthy control group,the proportion of peripheral blood mononuclear cells Th17 cells in the spondylitis group and the preoperative serum IL-17,IL-6,IL-23 levels increased(t/P=5.711/0.000,45.567/0.000,58.709/0.000,17.221/0.000),the proportion of peripheral blood mononuclear cells Th1 cells and serum IL-2,IFN-γlevels decreased(t/P=34.024/0.000,8.991/0.000,5.948/0.000).Compared with the non-recurring subgroup,the proportion of peripheral blood mononuclear cells Th17 cells and postoperative serum IL-17,IL-6,IL-23 levels in the recurring subgroup increased(t/P=5.509/0.000,37.286/0.000,46.074/0.000,18.982/0.000),the proportion of peripheral blood mononuclear cells Th1 cells and serum IL-2,IFN-γlevels decreased(t/P=15.960/0.000,3.450/0.001,2.712/0.000).The percentage of Th1,Th17 cells and IL-17,IL-6,IL-23,IL-2,IFN-γafter surgery are all risk factors for the recurrence of brucellosis spondylitis[OR(95%CI)=2.375(1.657~3.405),2.454(1.737~3.472),2.273(1.661~3.109),2.219(1.619~3.043),2.278(1.684~3.082),2.261(1.546~3.308),2.337(1.624~3.364)].Conclusion The proportion of Th17 cells in the peripheral blood of patients with recurrence after brucellosis spondylitis increases,while the proportion of Th1 cells decreases.The expression of Th1 and Th17 cells is closely related to the recovery of brucellosis spondylitis after surgery.
作者
贾永利
杨新明
张培楠
孟宪勇
胡长波
Jia Yongli;Yang Xinming;Zhang Peinan;Meng Xianyong;Hu Changbo(Department of Orthopedic Surgery,the First Hospital Affiliated to Hebei North University,Hebei Province,Zhangjiakou 075000,China)
出处
《疑难病杂志》
CAS
2021年第3期256-260,共5页
Chinese Journal of Difficult and Complicated Cases
基金
河北省医学科学研究课题计划项目(20190120)
河北省卫生健康技术研究暨成果转化重点项目(ZH2018014)
河北省医学适用技术跟踪项目(G2018074)
张家口市市级科技计划项目(17120010D)。
