摘要
目的探讨寿命蛋白(Mortalin)在子宫内膜癌(EC)中过表达的临床病理学意义。方法采用免疫组织化学EnVision法检测Mortalin蛋白在88例EC组织和37例正常子宫内膜组织中的表达,分析其过表达与EC临床病理特征之间的关系。应用UALCAN数据库分析Mortalin mRNA在EC组织中的表达水平,应用The Human Protein Atlas和The Cancer Genome Atlas(TCGA)数据库进行生存分析。结果Mortalin蛋白主要表达于EC细胞的细胞质中;在EC组织中,Mortalin蛋白的阳性率和强阳性率分别为88.6%(78/88)和56.8%(50/88),高于正常子宫内膜组织(P<0.05)。Mortalin蛋白的表达与EC患者的病理分级、TNM分期和淋巴结转移相关(P<0.05),但与年龄无关(P>0.05)。UALCAN数据库分析显示,EC组织中的Mortalin mRNA表达高于正常子宫内膜组织(P<0.05)。The Human Protein Atlas和TCGA数据库生存分析结果显示,Mortalin蛋白和mRNA高表达的EC患者总生存期低于Mortalin蛋白和mRNA低表达患者(P<0.05)。结论Mortalin的表达与EC的发生、发展及预后相关,其有望成为EC患者靶向治疗及预后评估的重要指标。
Objective To investigate the clinicopathological significance of the overexpression of Mortalin protein in endometrial carcinoma(EC).Methods Mortalin expression was evaluated in 88 EC samples and 37 paracarcinoma tissue samples by immunohistochemical(IHC)staining.The expression of Mortalin mRNA in EC tissues was analyzed by UALCAN database.Correlations between Mortalin overexpression and clinicopathological features of EC patients were analyzed.Overall survival rate of EC patients was performed based on The Human Protein Atlas and The Cancer Genome Atlas(TCGA)databases.Results Mortalin showed a cytoplasmic staining pattern in EC.The positive rate and strongly positive rate of Mortalin in EC were 88.6%(78/88)and 56.8%(50/88),which were both significantly higher compared with para-carcinoma tissues(P<0.05).Mortalin overexpression was positively correlated with the pathological grade,TNM stage and LN metastasis in EC(P<0.05),while it had no statistical significance with age(P>0.05).The UALCAN database noted that the expression of Mortalin mRNA was elevated in EC tissues compared with that observed in paracarcinoma tissue samples.Additionally,The Human Protein Atlas and TCGA database showed that the patients with high Mortalin expression had poorer prognosis than those with low Mortalin expression(P<0.05).Conclusion Mortalin high expression is significantly associated with tumorigenesis,cancer progression and the prognosis of EC patients,which may serve as an important indicator for target therapy and prognosis evaluation in EC.
作者
尹香琳
王馨悦
韩安娜
李楠
杨洋
Xiang-lin Yin;Xin-yueWang;Anna Han;Nan Li;Yang Yang(Yanbian University Medical College,Yanji,Jilin 133002,China;Wenzhou University,Wenzhou,Zhejiang 325035,China)
出处
《中国现代医学杂志》
CAS
北大核心
2021年第1期15-20,共6页
China Journal of Modern Medicine
基金
延边大学应用基础项目[No:延大科合字(2019)第20号]。
