摘要
在盆栽条件下,以耐冷性不同的2个小豆(Vigna angularis)品种龙小豆4号(耐冷型)和天津红(冷敏型)为材料,于苗期在人工气候室进行低温(4℃,持续12 h)及预喷施外源脱落酸(abscisic acid,ABA)处理,旨在研究苗期低温胁迫下外源ABA对小豆叶片逆境生理指标(H2O2、丙二醛(MDA)、抗氧化酶活性、可溶性物质)和产量因子的调控效应。结果表明:幼苗期低温处理12 h后,龙小豆4号和天津红小豆叶片H2O2含量分别提高34.82%和27.10%;MDA含量分别提高65.56%和83.79%;SOD和POD活性分别提高6.67%和18.48%、45.53%和102.44%;脯氨酸含量分别提高33.12%和24.41%。低温处理导致龙小豆4号百粒重下降2.58%,与喷施清水相比,低温处理12 h后天津红的单株荚数和百粒重分别下降18.55%和10.61%(P<0.05)。喷施外源ABA具有抵御低温的作用,外源ABA促进龙小豆4号和天津红叶片脯氨酸含量提高9.13%和19.76%;H2O2含量降低7.63%和8.36%;MDA含量降低10.18%和20.27%;SOD和POD活性分别提高9.06%和13.57%、8.26%和28.51%,同时百粒重分别提高8.56%和8.92%。低温胁迫下,不同品种小豆的生理应激存在显著差异;喷施外源ABA能够有效缓解低温胁迫对小豆的伤害。
In order to investigate the difference in the resistance physiology and yield of adzuki bean seedlings,and the mitigation effect of exogenous ABA pretreatment under low temperature,a pot experiment in this research was conducted in an artificial climate chamber.The crop cultivars including cold tolerant and cold sensitive separately were Longxiaodou 4(LXD 4)and Tianjinhong(TJH).The treatment of low temperature(4℃)and foliar spraying exogenous ABA in seedling period of adzuki bean were applied.The time of low temperature in the experiment last for 12 h.The purpose was to analyze the effect of exogenous ABA pretreatment under low temperature on the anti-stress physiological indexes(H2O2,malonaldehyde(MDA),antioxidant enzyme activity,soluble substances)and yield factors of adzuki bean.The results showed that the H2O2 content in leaves of LXD 4 and TJH increased by 34.82%and 27.10%after 12 h of low temperature treatment at the seedling stage.The MDA content increased by 65.56%and 83.79%;SOD and POD activities increased by 6.67%and 18.48%,45.53%and 102.44%;and the proline content increased by 33.12%and 24.41%,respectively.Low temperature treatment caused the 100-seed weight of LXD 4 to decrease significantly by 2.58%,and the number of pods and 100-seed weight of TJH decreased significantly by 18.55%and 10.61%(P<0.05),respectively.Exogenous ABA had the effect of resisting low temperature.Compared with water,spraying exogenous ABA increased the proline content of LXD 4 and TJH by 9.13%and 19.76%;MDA content decreased by 10.18%and 20.27%;SOD and POD activities increased by 9.06%and 13.57%,8.26%and 28.51%after 12 h of low temperature treatment.In addition,the yield component(100-seed weight)of LXD 4 and TJH increased 8.56%and 8.92%,respectively.This study concluded that there were significant differences in physiological responses among different adzuki bean varieties,and spraying exogenous ABA effectively relieved damage of low temperature stress to adzuki bean,which provides a theoretical basis for further study on the physiological mechanism against low temperature injury and increases the yield of adzuki bean under low temperature.
作者
项洪涛
李琬
郑殿峰
王诗雅
何宁
王曼力
王彤彤
杨纯杰
李博
XIANG Hongtao;LI Wan;ZHENG Dianfeng;WANG Shiya;HE Ning;WANG Manli;WANG Tongtong;YANG Chunjie;LI Bo(Institute of Crop Cultivation and Tillage, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150086, China;College of Coastal Agriculture Sciences, Guangdong Ocean University, Zhanjiang, Guangdong 524088, China;College of Agriculture, Heilongjiang Bayi Agriculture University, Daqing, Heilongjiang 163319, China)
出处
《干旱地区农业研究》
CSCD
北大核心
2020年第6期52-60,共9页
Agricultural Research in the Arid Areas
基金
黑龙江省农业科学院农业科技创新跨越工程杂粮杂豆科技创新专项(HNK2019CX05-12)
国家自然科学基金面上项目(31871576)
国家食用豆产业技术体系项目(CARS-08-04B)。
关键词
小豆幼苗
ABA
低温胁迫
生理指标
产量
adzuki bean seedlings
ABA
low temperature resistance
physiological index
yield
