摘要
基因组学的快速发展影响了医学所有领域。在输血医学中,基因分型技术的快速发展使其成为基于血清学方法检测血型的强力支持和辅助技术。在实际临床工作中,对于直接抗球蛋白试验阳性、多次输血或近期输血、自身免疫性溶血性贫血、自身存在多种抗体等情况的患者,以及胎儿或新生儿血型鉴定等困难时,特别是对没有商品化血清学试剂的稀有血型抗原进行检测时,血型基因检测技术是精准鉴定血型的重要手段。不同通量的血型基因检测技术存在各自的适用范围,高通量的基因筛查平台展示了其在基因检测技术广阔的应用前景。现阶段虽然分子生物学技术的应用仍然存在一定的局限性,但是随着基因型预测血型表型能力的提高,对具有临床意义的血型抗原进行高分辨率的分型工作将会越来越普及。
Genomics is impacting all fields of medicine.In transfusion medicine,the rapid development of genotyping has made itself a powerful support and additional technique for serologicalblood typing.In clinical practice,DNA-based genotyping is the key to accurate identification of blood group in such cases as positive direct antiglobulin test,multiply transfusions or recently received transfusions,autoimmune hemolytic anemia,multiple antibodies,fetal/neonatal blood group serologic untypable,especially rare blood type with no commercial serologi ckits available.Molecular blood groupings with different throughputs have their particular scope of application,and the high-throughput gene screening platform has exhibited its promising application in genotyping.High-resolution typing for blood group antigens with clinical significance will becoming more and more popular as the accuracy prediction of phenotype by molecular blood groupings improved,though it has some limitations at present.
作者
冯晨晨
陈青
FENG Chenchen;CHEN Qing(Jiangsu Province Blood Center,Nanjing 210042,China;Center for Global Health,School ofPublic Health,Nanjing Medical University)
出处
《中国输血杂志》
CAS
2020年第12期1231-1234,共4页
Chinese Journal of Blood Transfusion
基金
江苏省“十三五”期间“科教强卫工程”医学重点人才(ZDRCB2016010)
江苏省重点研发计划社会发展项目(BE2015717)
江苏省高层次卫生人才“六个一工程”拔尖人才项目(LGY2017095)
江苏省第五期“333高层次人才培养工程”(2016-III-3344)。
关键词
血型鉴定
基因组学
分子生物学技术
通量
blood typing
genomics
molecular biology technology
throughput
