摘要
目的探讨高强度间歇训练(high-intensity interval training,HIIT)对饮食诱导非酒精性脂肪肝(non-alcoholic fatty liver disease,NAFLD)脂质代谢的作用及其机制。方法雄性SPF级SD大鼠60只,随机分组,正常对照组(C)12只,普通饲料喂养不运动;其余48只高脂饲料喂养8周,造模NAFLD成型后,随机分为:模型组(M)、高脂饲料+运动组(HE)、普通饲料+运动组(GE)每组各12只,运动组以70%Vo2max强度进行高强度间歇训练(HIIT)和饮食干预。6周后,比较各组大鼠血脂变化;血清、肝组织超氧化物歧化酶(T-SOD)活性、丙二醛(MDA)含量和观察肝组织病理学改变。结果与C组、M组相比HE和GE组的肝脏指数明显增大,差异有统计学意义(P<0.05);经运动干预后,HE和GE组TG、LDL-C水平显著降低,HDL-C水平显著升高,差异有统计学意义(P<0.05);与M组相比,C、GE组游离脂肪酸(FFA)、血清胰岛素(Flns)水平均有下降,差异有统计学意义(P<0.01);C、HE、GE组FFA浓度水平与M组相比均有下降,差异有统计学意义(P<0.05);与C、M组相比HE组血清、肝组织的MDA水平差异有统计学意义(P<0.05);与C、M组相比GE组血清、肝组织的MDA水平差异有统计学意义(P<0.05);运动干预可以提高T-SOD活性水平。M组血清、肝组织T-SOD与HE、GE组差异均有均具有统计学意义(P<0.05)结论HIIT可以改善高脂饮食大鼠血脂代谢异常,提高肝组织T-SOD活性,减轻肝脏脂质过氧化反应。HIIT和饮食联合干预在调节血脂、抗NAFLD形成的效果较明显。
Objective To explore the effect of high-intensity interval training(HIIT)on lipid metabolism of non-alcoholic fatty liver disease(NAFLD)and its mechanism.Methods A total of 60 male SPF Sprague-Dawley rats were ranmaining 48 rats were given high-fat diet for 8 weeks to establish a NAFLD rat model.After successful modeling,48 rats were randomly divided into model group(M),high-fat feed+exercise group(HE),and normal feed+exercise group(GE),12 rats in each group.The exercise group received high-intensity interval training(HIIT)and diet intervention at 70%Vo2max intensity.After 6 weeks,the lipid changes in each group of rats were examined and the serum and liver tissue superoxide dismutase(T-SOD)activity and malondialdehyde(MDA)levels were measured and liver histopathological changes were observed.Results Compared with group C and MC group,the liver index of HE and GE group were significantly increased(P<0.05).After the exercise intervention,TG and LDL-C levels were significantly lower and HDL-C levels were significantly higher in the HE and GE groups(P<0.05).Compared with the M group,the FFA and Flns levels in the C and GE groups were significantly decreased(P<0.01),the FFA concentration levels in the C,HE,and GE groups were significantly decreased(P<0.05).Compared with the C and M groups,the serum and liver tissue MDA levels of the HE and GE group were significantly decreased(P<0.05).In addition,the T-SOD of serum and liver tissue in group M,HE and GE groups were significantly increased(P<0.05).Conclusion HIIT can increase liver tissue T-SOD activity,and reduce liver lipid peroxidation.The combined intervention of HIIT and diet is more effective in regulating blood lipids and anti-NAFLD formation.
作者
姜涛
刘敏娟
闫冬
JIANG Tao;LIU Minjuan;YAN Dong(Department of Physical Education,Xinjiang Medical University,Xinjiang Medical University Urumqi 830011,China;School of Pharmacy,Xinjiang Medical University Urumqi 830011,China)
出处
《新疆医科大学学报》
CAS
2021年第1期44-48,54,共6页
Journal of Xinjiang Medical University
基金
新疆维吾尔自治区自然科学基金(2017D01C208)。
关键词
高强度间歇训练
饮食
非酒精性脂肪肝
high-intensity interval training
diet
non-alcoholic fatty liver
