摘要
目的分析子痫前期患者胎盘生长因子及可溶性血管内皮生长因子受体1(sFlt-1)水平变化情况,为子痫前期患者的病理机制研究与治疗提供依据。方法选取2018年2月至2020年5月潍坊市妇幼保健院收治的90例妊娠期妇女为研究对象,其中30例轻度子痫前期纳入A组,30例重度子痫前期纳入B组,30例正常妊娠妇女纳入对照组,检查三组妊娠期妇女的胎盘生长因子(PLGF)及sFlt-1水平,同时通过逆转录聚合酶链式反应(RT-PCR)技术与蛋白质印迹法(Western Blot)测定不同妊娠期妇女胎盘组织中PLGF mRNA、sFlt-1 mRNA以及蛋白表达情况,对三组不同妊娠期妇女各项资料予以数据分析。结果A组、B组胎盘组织中的PLGF mRNA小于对照组,A组大于B组;A组、B组胎盘组织中的sFlt-1 mRNA大于对照组,A组小于B组,差异有统计学意义(P<0.05)。A组、B组胎盘组织中的PLGF蛋白水平小于对照组,A组大于B组;A组、B组胎盘组织中的sFlt-1蛋白大于对照组,A组小于B组,差异有统计学意义(P<0.05)。A组、B组血清PLGF小于对照组,A组大于B组,A组、B组血清sFlt-1大于对照组,A组小于B组,差异有统计学意义(P<0.05)。结论胎盘生长因子及sFlt-1水平可能与子痫前期病情发生以及疾病进展有着密切关系。
Objective To analyze the changes of placental growth factor and soluble vascular endothelial growth factor receptor 1(sFlt-1)levels in patients with preeclampsia,so as to provide evidence for pathological mechanism research and treatment of preeclampsia patients.Methods Totally 90 pregnant women admitted to Weifang Maternal and Child Health Hospital from February 2018 to May 2020 were selected as study subjects,of whom 30 with mild preeclampsia were included in group A,30 with severe preeclampsia were included in group B,and 30 normal pregnant women were included in the control group,and levels of placental growth factor(PLGF)and sFlt-1 were examined in three pregnant women.In parallel,PLGF mRNA and sFlt-1 mRNA as well as protein expression were measured by reverse transcription polymerase chain reaction(RT-PCR)with Western blot analysis in placental tissues from women at different gestational ages,with data available for all three groups of women at different gestational ages.Results PLGF mRNA in the placental tissue of groups A and B was less than that in the control group and group A was greater than that in group B.sFlt-1 mRNA in the placental tissue of groups A and B was greater than that in the control group and group A was less than that in group B,with significant differences(P<0.05).The PLGF protein level in the placental tissue of groups A and B was less than that in the control group and group A was greater than that in group B.The sFlt-1 protein in the placental tissue of groups A and B was greater than that in the control group and group A was less than that in group B,with significant differences(P<0.05).The serum PLGF in groups A and B was less than that in the control group,group A was greater than group B,the serum sFlt-1 in groups A and B was greater than that in the control group,and group A was less than that in group B,with significant differences(P<0.05).Conclusions Levels of placental growth factor and soluble vascular endothelial growth factor receptor 1 may be strongly associated with the development of preeclampsia as well as with disease progression.
作者
宋婷婷
SONG Tingting(Weifang Maternal and Child Health Hospital,Weifang,Shandong 261000,China)
出处
《大医生》
2020年第19期121-123,共3页
Doctor
关键词
子痫前期
胎盘生长因子
可溶性血管内皮生长因子受体1
病理机制
preeclampsia
placental growth factor
soluble vascular endothelial growth factor receptor 1
pathological mechanism
