摘要
春小麦的苗期生长受到干旱的严重影响,进而影响其产量和品质。为了明确响应干旱胁迫的关键表达基因,本研究以PEG胁迫和相应对照春小麦苗期叶片为材料构建转录组测序文库,利用Illumina HiSeq^TM 2000进行测序,采用FPKM法计算基因表达量,对差异表达基因进行功能分类和KEGG通路分析。结果表明,样品测序均得到6.4 G以上的碱基,每组样品Q30碱基百分比均大于88.73%。最终结果筛选到差异表达基因1 217个,其中410个上调表达基因,807个下调表达基因,显著富集到谷胱甘肽和苯丙烷类等代谢途径。本研究结果为小麦等作物抗旱基因的挖掘和功能研究奠定了良好的基础。
Drought at seedling stage has a serious impact on the growth and development of spring wheat, further affecting the yield and quality. In order to identify the key expression genes in response to drought stress, a transcriptome sequencing library from the leaves of Spring Wheat Seedlings under PEG stress and corresponding control was constructed. Illumina HiSeq^TM 2000 was used to sequence the genes, FPKM method was used to calculate the gene expression level, functional classification of differentially expressed genes and KEGG pathway analysis were carried out. The results showed that each sample obtained more than 6.4 G bases, and the percentage of Q30 bases in each sample was more than 88.73%. A total of 1 217 differentially expressed genes were screened,including 410 up-regulated genes and 807 down-regulated genes, which were significantly enriched in metabolic pathways such as glutathione metabolism and phenylpropane biosynthesis. The results laid a good foundation for the research of drought resistance genes in wheat and other crops.
作者
杨文君
叶景秀
李生荣
马宏
Yang Wenjun;Ye Jingxiu;Li Shengrong;Ma Hong(Academy of Agriculture and Forestry Science of Qinghai University,Xining,810016)
出处
《分子植物育种》
CAS
CSCD
北大核心
2020年第24期7959-7965,共7页
Molecular Plant Breeding
基金
青海省(应用)基础研究计划项目(2013-Z-721)
青海省科技厅科技平台建设项目(2017-ZJ-Y41)
国家重点实验室培育基地青海省高原作物种质资源创新与利用重点实验室开放课题任务书(2014-02)共同资助。
关键词
PEG胁迫
春小麦
苗期
转录组
PEG stress
Spring wheat
Seedling stage
Transcriptome
