摘要
目的:了解寻常痤疮患者中痤疮丙酸杆菌耐药情况,并探讨其可能的分子生物学机制。方法:选择18例寻常痤疮患者炎症性皮损,厌氧条件下分离、培养和鉴定获得12个痤疮丙酸杆菌临床株。采用纸片法对红霉素、克林霉素、甲硝唑和四环素4种常用抗生素进行体外药敏试验。根据药敏试验结果,选择对红霉素耐药菌株并提取细菌DNA,对23S rRNA基因扩增和测序,分析其核苷酸序列变化。结果:所有菌株均对四环素高度敏感而对克林霉素和甲硝唑均耐药,11个临床株对红霉素耐药,仅一株中度敏感。对12株23S rRNA序列经PCR测序后发现共7株发生第1392位A→G、第1393位A→G,第1518位A→G、第1533位T→C、第1569位A→G、第1814位G→A、第1839位G→A、第1946位T→C、第1974位A→G、第2034位T→C、第2058位A→T、第2273位A→G和第2513位T→C碱基变化,其余5株的核苷酸序列没有变化。结论:结合以前关于PA耐药基因的分子检测结果,除了已经报道的第2058位A→T变化外,本研究发现多个23S rRNA新突变位点以及同一菌株发生多位点突变,而部分菌株的核苷酸序列没有变化,这些新突变位点以及同一菌株多位点突变与痤疮丙酸杆菌对红霉素耐药性的关联性有待进一步证实,并可能存在其他机制。
Objective:To investigate propionibacterium acne resistance in patients with acne vulgaris and explore its possible molecular mechanism.Methods:Twelve clinical strains of propionibacterium acne were isolated and identified from eighteen patients with acne vulgaris.The antibiotic sensitivity of erythromycin,clindamycin,metronidazole and tetracycline was tested by disk method in vitro.Bacteria DNA was extracted from erythromycin-resistant strains.The 23SrRNA gene was amplified and sequenced.The nucleotide sequences were analyzed.Results:All clinical strains were highly sensitive to tetracycline and resistant to clindamycin and metronidazole.Eleven clinical strains were erythromycin-resistant,and only one strain was mildly sensitive to erythromycin.The 23SrRNA of 12 clinical strains were sequenced.Seven strains were found to have nucleotide changes with 1392A→G,1393A→G,1518A→G,1533T→C,1569A→G,1814G→A,1839G→A,1946T→C,1974A→G,2034T→C,2058A→T,2273A→G and 2513T→C alternations while no nucleotides alternations were found in the other 5 strains.Conclusion:Apart from the reported 2058 A/T change,multiple 23SrRNA new mutation sites and several mutations in the same strain were found,while no nucleotide changes were found in some strains.The relationship between erythromycin-resistance and these new mutation sites or multi-site mutation in the same strain needs to be confirmed.Furthermore,this study suggests that there may be other molecular mechanisms in eryhthrimycin-resistance of propionibacterium acne in patients with acne vulgaris.
作者
郭娴菲
尹兴平
黄海峰
赵琪
毕鸣晔
GUO Xian-fei;YIN Xing-ping;HUANG Hai-feng;ZHAO Qi;BI Ming-ye(Department of Dermatology,Wuxi People’s Hospital Affiliated to Nwijing Medical University,Nanjing 214023,China)
出处
《临床皮肤科杂志》
CAS
CSCD
北大核心
2020年第12期717-722,共6页
Journal of Clinical Dermatology
