摘要
Protecting the quality of lake watersheds by preventing and reducing their contamination is an effective approach to ensure the sustainability of the drinking water supply.In this study,acute toxicity assessment was conducted on the basis of acute bioluminescence inhibition assay using the marine bacterium Vibrio fischeri as the test organism and Luoma Lake drinking water source in East China as the research target.The suitable ranges of environmental factors,including pH value,organic matter,turbidity,hardness,and dissolved oxygen of water samples were evaluated for the toxicity testing of bioluminescent bacteria.The physicochemical characteristics of water samples at the selected 43 sites of Luoma Lake watershed were measured.Results showed that the variations in pH value(7.31-8.41),hardness(5-20°d)and dissolved oxygen(4.44-11.03 mg/L)of Luoma Lake and its main inflow and outflow rivers had negligible impacts on the acute toxicity testing of V.fischeri.The luminescence inhibition rates ranged from-11.21%to 10.80%at the 43 sites.Pearson's correlation analysis in the experiment revealed that temperature,pH value,hardness,and turbidity had no correlation with luminescence inhibition rate,whereas dissolved oxygen showed a weak statistically positive correlation with a Pearson correlation coefficient of 0.455(p<0.05).
基金
This research was supported by Provincial Environmental Protection Research Project of Jiangsu(No.2018002)。
