摘要
为快速检测牛乳中残留的四环素和青霉素,应用胶体金免疫层析法,建立可以同时对四环素和青霉素进行检测的双重胶体金试纸条检测方法。结果表明:胶体金侧向免疫层析法用于检测牛乳中的四环素和青霉素,检出限均为2 ng/mL,检测时间10 min,方法特异性较好,与牛乳中常见的其他检测物无交叉反应。该方法简单可靠,为现场快速检测牛乳中的抗生素残留提供一种简单有效的方法。
A rapid double colloidal gold immunochromatographic test strip was developed for the simultaneous detection of residual tetracycline and penicillin in milk samples.The results showed that the detection limit for both tetracycline and penicillin was 2 ng/mL,and the detection time was 10 min.This method had good specificity,and exhibited no crossreaction with other analytes commonly found in milk.The method is simple and reliable,and provides a simple and effective tool for the rapid and on-site detection of antibiotic residues in milk.
作者
吴玉晗
陈伟
WU Yuhan;CHEN Wei(School of Food and Biological Engineering,Hefei University of Technology,Hefei 230009,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2020年第24期281-286,共6页
Food Science
基金
“十三五”国家重点研发计划重点专项(2018YFC1603606)。
关键词
牛乳
四环素
青霉素
多重
快速检测
milk
tetracycline
penicillin
multiple
rapid detection
